Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody (PA516891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC (31635) was used at a concentration of 4µg/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of Alpha tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Rhodamine Phalloidin (R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:50 - 1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31635 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31635 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 9.3 ug/mg; > 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.5 ml of distilled water (1.5 mg/ml after restoration).
Country of Origin: USA
Thermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of Peroxisome Proliferator-Activated Receptor alpha (PPAR¿) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells.
31635 was used in immunohistochemistry - paraffin section to determine the relationship with aryl hydrocarbon receptor interacting protein (AIP) and in vitor effects of fenofibrate in GH3 cells to study expression of peroxisome proliferator-activated rec
|Rotondi S,Modarelli A,Oliva MA,Rostomyan L,Sanita P,Ventura L,Daly AF,Esposito V,Angelucci A,Arcella A,Giangaspero F,Beckers A,Jaffrain-Rea ML||Molecular and cellular endocrinology (426:61)||2016|
|Not Applicable||Not Cited||
Prolyl oligopeptidase colocalizes with ¿-synuclein, ß-amyloid, tau protein and astroglia in the post-mortem brain samples with Parkinson's and Alzheimer's diseases.
31635 was used in western blot to study the potential involvement of prolyl oligopeptidase in neurodegenerative diseases by monitoring its colocalization with pathological proteins in Parkinson's and Alzheimer's diseases
|Hannula MJ,Myöhänen TT,Tenorio-Laranga J,Männistö PT,Garcia-Horsman JA||Neuroscience (242:140)||2013|
Catechol-O-methyltransferase (COMT) protein expression and activity after dopaminergic and noradrenergic lesions of the rat brain.
31635 was used in immunohistochemistry to study the effects of dopaminergic and noradrenergic lesions on COMT protein and activity levels in rat brain
|Schendzielorz N,Oinas JP,Myöhänen TT,Reenilä I,Raasmaja A,Männistö PT||PloS one (8:null)||2013|