|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.4, with 1% BSA, 40% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Western Blot (WB)||1:20-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||1 µg/ml||Zinc protects renal function during cadmium intoxication in the rat.||Jacquillet G,Barbier O,Cougnon M,Tauc M,Namorado MC,Martin D,Reyes JL,Poujeol P||American journal of physiology. Renal physiology (290:F127)||2006|
Epidermal transit of replication-arrested, undifferentiated keratinocytes in UV-exposed XPC mice: an alternative to in situ apoptosis.
65-6111 was used in immunohistochemistry - paraffin section to examine the effects of UV exposure in Xpc knockout mice
|Stout GJ,Westdijk D,Calkhoven DM,Pijper O,Backendorf CM,Willemze R,Mullenders LH,de Gruijl FR||Proceedings of the National Academy of Sciences of the United States of America (102:18980)||2005|
Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells.
65-6111 was used in immunocytochemistry to characterize the nuclear functions of ZO-2
|Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L||Experimental cell research (297:247)||2004|
Quantitative imaging of tyrosine hydroxylase and calmodulin in the human brain.
65-6111 was used in immunocytochemistry to measure tyrosine hydroxylase and calmodulin in adult normal postmortem human brains
|Sutoo D,Akiyama K,Yabe K||Journal of neuroscience research (63:369)||2001|
||Immunocytochemical evidence for a possible role of cross-linked keratinocyte envelopes in stratum corneum cohesion.||Haftek M,Serre G,Mils V,Thivolet J||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (39:1531)||1991|
|Not Applicable||6 µg/ml||
The renal segmental distribution of claudins changes with development.
65-6111 was used in immunohistochemistry (frozen) to examine the differential expression of occludin and several claudins in isolated renal tubules from newborn and adult rabbits.
|Reyes JL,Lamas M,Martin D,del Carmen Namorado M,Islas S,Luna J,Tauc M,González-Mariscal L||Kidney international (62:476)||2002|
|Not Applicable||Not Cited||Immunocytochemical evidence for a possible role of cross-linked keratinocyte envelopes in stratum corneum cohesion.||Haftek M,Serre G,Mils V,Thivolet J||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (39:1531)||1991|