Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with ABfinity TM Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product # 702211, 2 µg/ml) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Rabbit IgG (H+L) Secondary Antibody, AP (Product # 65-6122) at dilutions 1:1,000 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 25 kDa band corresponding to PRDX6 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product #WP20003).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||0.05M tris, pH 7.5, with 50% glycerol, 1mM MgCl2, 1% BSA, 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1,000|
|Western Blot (WB)||1:1,000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dermatophagoides-induced interleukin-10 production by peripheral blood lymphocytes from patients with asthma in remission.
65-6122 was used in ELISA to investigate the role of IL-10 in the development of tolerance to Dermatophagoides farinae in asthma patients in remission
|Noma T,Sugawara Y,Ogawa N,Saeki T,Yamaguchi K,Kawano Y||Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology (15:459)||2004|
Pattern of cytokine production by T cells from adolescents with asthma in remission, after stimulation with Dermatophagoides farinae antigen.
65-6122 was used in immunocytochemistry to study Dermatophagoides farinae-induced allergic responses in patients with asthma and compare them to those in remission.
|Noma T,Yoshizawa I,Kou K,Nakajima T,Kawano Y,Itoh M,Ichikawa K,Mukouyama T,Baba M,Yata J||Pediatric research (38:187)||1995|