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Immunofluorescence was performed on mouse E10.5 embryo trunk at the forelimb level. Embryos were fixed with 4%PFA in 0.1M phosphate buffer, pH 7.2 at 4C for 30 minutes, processed for cryoprotection in 20% sucrose, and embedded in OCT compound for cryosectioning at 10 um thickness. Following sectioning, tissues were washed in PBS with 0.1% Triton-X 100 (PBT), blocked in 1% goat serum in PBT for 30 minutes at room temperature, and then probed with a Pax3 polyclonal antibody (Product # PA1-107) at a concentration of 1ug/ml in blocking buffer overnight at 4C. Tissues were washed extensively with PBT, and detection was performed using an Alexa Fluor 568-conjugated goat anti-rabbit IgG secondary antibody (red) at a dilution of 1:1000 in blocking buffer. Nuclei (blue) were stained with DAPI. Note: Staining was observed in the dorsal neural tube (symmetric two halves on the left side of the image), the dermomyotome/myotome (middle streak between symmetric halves), and limb muscle progenitors (scattered cells to the right side of the image). Data supplied courtesy of the Innovator's Program.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 568|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
To minimize cross-reactivity, these goat anti-rabbit IgG whole antibodies have been cross-adsorbed against human IgG, human serum, mouse IgG, mouse serum, and bovine serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 568 dye is a bright, orange/red-fluorescent dye with excitation ideally suited to the 568 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.
Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.
Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Applied stretch initiates directional invasion through the action of Rap1 GTPase as a tension sensor.
A-11011 was used in immunocytochemistry to elucidate initiation of directional invasion via the action of Rap1 GTPase as a tension sensor by applied stretch
|Freeman SA,Christian S,Austin P,Iu I,Graves ML,Huang L,Tang S,Coombs D,Gold MR,Roskelley CD||Journal of cell science (130:152)||2017|
Nanos-mediated repression of hid protects larval sensory neurons after a global switch in sensitivity to apoptotic signals.
A-11011 was used in immunocytochemistry to study protection of larval sensory neurons after a global switch in sensitivity to apoptotic signals by nanos-mediated repression of hid
|Bhogal B,Plaza-Jennings A,Gavis ER||Development (Cambridge, England) (143:2147)||2016|
CCDC115 Deficiency Causes a Disorder of Golgi Homeostasis with Abnormal Protein Glycosylation.
A-11011 was used in immunocytochemistry to study the cause of a golgi homeostasis disorder with abnormal protein glycosylation due to CCDC115 deficiency
|Jansen JC,Cirak S,van Scherpenzeel M,Timal S,Reunert J,Rust S,Pérez B,Vicogne D,Krawitz P,Wada Y,Ashikov A,Pérez-Cerdá C,Medrano C,Arnoldy A,Hoischen A,Huijben K,Steenbergen G,Quelhas D,Diogo L,Rymen D,Jaeken J,Guffon N,Cheillan D,van den Heuvel LP,Maeda Y,Kaiser O,Schara U,Gerner P,van den Boogert MA,Holleboom AG,Nassogne MC,Sokal E,Salomon J,van den Bogaart G,Drenth JP,Huynen MA,Veltman JA,Wevers RA,Morava E,Matthijs G,Foulquier F,Marquardt T,Lefeber DJ||American journal of human genetics (98:310)||2016|
Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
A-11011 was used in immunocytochemistry to utilize immunodetection of planarian neoblasts and tracing of BrdU-labeled cells after partial irradiation to analyze stem cell motility in vivo
|Tasaki J,Uchiyama-Tasaki C,Rouhana L||Methods in molecular biology (Clifton, N.J.) (1365:323)||2015|
Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures.
A-11011 was used in immunocytochemistry to use a high-throughput 3D hyaluronic acid hydrogel coculture of cancer cells with stromal cells to evaluate chemotherapeutics
|Engel BJ,Constantinou PE,Sablatura LK,Doty NJ,Carson DD,Farach-Carson MC,Harrington DA,Zarembinski TI||Advanced healthcare materials (4:1664)||2015|
A novel dual-flow bioreactor simulates increased fluorescein permeability in epithelial tissue barriers.
A-11011 was used in immunocytochemistry to describe a novel two-chamber modular bioreactor for dynamic in vitro studies of epithelial cells
|Giusti S,Sbrana T,La Marca M,Di Patria V,Martinucci V,Tirella A,Domenici C,Ahluwalia A||Biotechnology journal (9:1175)||2014|
|Not Applicable||Not Cited||
The chromatin remodeling protein BRG1 modulates BRCA1 response to UV irradiation by regulating ATR/ATM activation.
A-11011 was used in immunocytochemistry to propose that BRG1 modulates BRCA1 response to UV irradiation by regulating ATM/ATR activation
|Zhang L,Chen H,Gong M,Gong F||Frontiers in oncology (3:null)||2013|
|Not Applicable||Not Cited||Characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials.||Yang MH,Yuan SS,Chung TW,Jong SB,Lu CY,Tsai WC,Chen WC,Lin PC,Chiang PW,Tyan YC||BioMed research international (2014:null)||2014|
|Not Applicable||Not Cited||Intracellular trafficking of Clostridium perfringens iota-toxin b.||Nagahama M,Umezaki M,Tashiro R,Oda M,Kobayashi K,Shibutani M,Takagishi T,Ishidoh K,Fukuda M,Sakurai J||Infection and immunity (80:3410)||2012|
|Not Applicable||Not Cited||Alteration in neonatal nutrition causes perturbations in hypothalamic neural circuits controlling reproductive function.||Caron E,Ciofi P,Prevot V,Bouret SG||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:11486)||2012|
|Not Applicable||Not Cited||Purified human pancreatic duct cell culture conditions defined by serum-free high-content growth factor screening.||Hoesli CA,Johnson JD,Piret JM||PloS one (7:null)||2012|
|Not Applicable||Not Cited||
Reduced expression of SynGAP, a neuronal GTPase-activating protein, enhances capsaicin-induced peripheral sensitization.
A-11011 was used in immunohistochemistry to study the role of SynGAP in peripheral sensitization and pain
|Duarte DB,Duan JH,Nicol GD,Vasko MR,Hingtgen CM||Journal of neurophysiology (106:309)||2011|
|Not Applicable||Not Cited||
ON and OFF pathways in Drosophila motion vision.
A-11011 was used in immunohistochemistry to test how L1-L5 contribute to visual motion detection in Drosophila melanogaster.
|Joesch M,Schnell B,Raghu SV,Reiff DF,Borst A||Nature (468:300)||2010|
|Not Applicable||Not Cited||Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties.||Zorzi P,Aplin AC,Smith KD,Nicosia RF||Journal of leukocyte biology (88:1051)||2010|
|Not Applicable||Not Cited||Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.||Robertson FM,Ogasawara MA,Ye Z,Chu K,Pickei R,Debeb BG,Woodward WA,Hittelman WN,Cristofanilli M,Barsky SH||Journal of biomolecular screening (15:820)||2010|
|Not Applicable||Not Cited||Systems survey of endocytosis by multiparametric image analysis.||Collinet C,Stöter M,Bradshaw CR,Samusik N,Rink JC,Kenski D,Habermann B,Buchholz F,Henschel R,Mueller MS,Nagel WE,Fava E,Kalaidzidis Y,Zerial M||Nature (464:243)||2010|
|Not Applicable||Not Cited||Quantification of host-microbe interactions by automated fluorescence microscopy.||Haghighat AC,Seveau S||Journal of immunological methods (352:186)||2010|
|Not Applicable||Not Cited||Mechanism for amyloid precursor-like protein 2 enhancement of major histocompatibility complex class I molecule degradation.||Tuli A,Sharma M,Capek HL,Naslavsky N,Caplan S,Solheim JC||The Journal of biological chemistry (284:34296)||2009|
|Not Applicable||Not Cited||Induction of glutathione-dependent DNA double-strand breaks by the novel anticancer drug brostallicin.||Guirouilh-Barbat J,Zhang YW,Pommier Y||Molecular cancer therapeutics (8:1985)||2009|
|Not Applicable||Not Cited||Subcellular localization of the antidepressant-sensitive norepinephrine transporter.||Matthies HJ,Han Q,Shields A,Wright J,Moore JL,Winder DG,Galli A,Blakely RD||BMC neuroscience (10:null)||2009|
|Not Applicable||Not Cited||T cell antigen receptor signaling and immunological synapse stability require myosin IIA.||Ilani T,Vasiliver-Shamis G,Vardhana S,Bretscher A,Dustin ML||Nature immunology (10:531)||2009|
|Not Applicable||Not Cited||Tuning of synapse number, structure and function in the cochlea.||Meyer AC,Frank T,Khimich D,Hoch G,Riedel D,Chapochnikov NM,Yarin YM,Harke B,Hell SW,Egner A,Moser T||Nature neuroscience (12:444)||2009|
|Not Applicable||Not Cited||Protein synthesis in distal axons is not required for growth cone responses to guidance cues.||Roche FK,Marsick BM,Letourneau PC||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:638)||2009|
|Not Applicable||Not Cited||Endophilin B1 as a novel regulator of nerve growth factor/ TrkA trafficking and neurite outgrowth.||Wan J,Cheung AY,Fu WY,Wu C,Zhang M,Mobley WC,Cheung ZH,Ip NY||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:9002)||2008|
|Not Applicable||Not Cited||Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands.||Peshenko IV,Olshevskaya EV,Dizhoor AM||The Journal of biological chemistry (283:21747)||2008|
|Not Applicable||Not Cited||HCO3(-) secretion by murine nasal submucosal gland serous acinar cells during Ca2+-stimulated fluid secretion.||Lee RJ,Harlow JM,Limberis MP,Wilson JM,Foskett JK||The Journal of general physiology (132:161)||2008|
|Not Applicable||Not Cited||Detection of de- and hyperpolarization of mitochondria of cultured astrocytes and neurons by the cationic fluorescent dye rhodamine 123.||Kahlert S,Zündorf G,Reiser G||Journal of neuroscience methods (171:87)||2008|
|Not Applicable||Not Cited||Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.||Damiani D,Alexander JJ,O'Rourke JR,McManus M,Jadhav AP,Cepko CL,Hauswirth WW,Harfe BD,Strettoi E||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:4878)||2008|
|Not Applicable||Not Cited||Methods for assessing autophagy and autophagic cell death.||Tasdemir E,Galluzzi L,Maiuri MC,Criollo A,Vitale I,Hangen E,Modjtahedi N,Kroemer G||Methods in molecular biology (Clifton, N.J.) (445:29)||2008|
|Not Applicable||Not Cited||Quantum dot-induced epigenetic and genotoxic changes in human breast cancer cells.||Choi AO,Brown SE,Szyf M,Maysinger D||Journal of molecular medicine (Berlin, Germany) (86:291)||2008|
|Not Applicable||Not Cited||Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart.||Ott HC,Matthiesen TS,Goh SK,Black LD,Kren SM,Netoff TI,Taylor DA||Nature medicine (14:213)||2008|
|Not Applicable||Not Cited||Limitations of green fluorescent protein as a cell lineage marker.||Swenson ES,Price JG,Brazelton T,Krause DS||Stem cells (Dayton, Ohio) (25:2593)||2007|
|Not Applicable||Not Cited||Characterization of SynCAM surface trafficking using a SynCAM derived ligand with high homophilic binding affinity.||Breillat C,Thoumine O,Choquet D||Biochemical and biophysical research communications (359:655)||2007|
|Not Applicable||Not Cited||Subcellular localization and physiological significance of intracellular mannan-binding protein.||Nonaka M,Ma BY,Ohtani M,Yamamoto A,Murata M,Totani K,Ito Y,Miwa K,Nogami W,Kawasaki N,Kawasaki T||The Journal of biological chemistry (282:17908)||2007|
|Not Applicable||Not Cited||A putative Src homology 3 domain binding motif but not the C-terminal dystrophin WW domain binding motif is required for dystroglycan function in cellular polarity in Drosophila.||Yatsenko AS,Gray EE,Shcherbata HR,Patterson LB,Sood VD,Kucherenko MM,Baker D,Ruohola-Baker H||The Journal of biological chemistry (282:15159)||2007|
|Not Applicable||Not Cited||Specialized inhibitory synaptic actions between nearby neocortical pyramidal neurons.||Ren M,Yoshimura Y,Takada N,Horibe S,Komatsu Y||Science (New York, N.Y.) (316:758)||2007|
|Not Applicable||Not Cited||Extracellular interactions between GluR2 and N-cadherin in spine regulation.||Saglietti L,Dequidt C,Kamieniarz K,Rousset MC,Valnegri P,Thoumine O,Beretta F,Fagni L,Choquet D,Sala C,Sheng M,Passafaro M||Neuron (54:461)||2007|
|Not Applicable||Not Cited||Jagged-mediated Notch signaling maintains proliferating neural progenitors and regulates cell diversity in the ventral spinal cord.||Yeo SY,Chitnis AB||Proceedings of the National Academy of Sciences of the United States of America (104:5913)||2007|
|Not Applicable||Not Cited||Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.||Yamazaki T,Yamagata K,Baba T||Developmental biology (304:409)||2007|
|Not Applicable||Not Cited||Phospholipid biosynthesis program underlying membrane expansion during B-lymphocyte differentiation.||Fagone P,Sriburi R,Ward-Chapman C,Frank M,Wang J,Gunter C,Brewer JW,Jackowski S||The Journal of biological chemistry (282:7591)||2007|
|Not Applicable||Not Cited||AP1B sorts basolateral proteins in recycling and biosynthetic routes of MDCK cells.||Gravotta D,Deora A,Perret E,Oyanadel C,Soza A,Schreiner R,Gonzalez A,Rodriguez-Boulan E||Proceedings of the National Academy of Sciences of the United States of America (104:1564)||2007|
|Not Applicable||Not Cited||p53 mediates nontranscriptional cell death in dopaminergic cells in response to proteasome inhibition.||Nair VD,McNaught KS,González-Maeso J,Sealfon SC,Olanow CW||The Journal of biological chemistry (281:39550)||2006|
|Not Applicable||Not Cited||NMDA receptor surface mobility depends on NR2A-2B subunits.||Groc L,Heine M,Cousins SL,Stephenson FA,Lounis B,Cognet L,Choquet D||Proceedings of the National Academy of Sciences of the United States of America (103:18769)||2006|
|Not Applicable||Not Cited||Regional variations in the glial influence on synapse development in the mouse CNS.||Steinmetz CC,Buard I,Claudepierre T,Nägler K,Pfrieger FW||The Journal of physiology (577:249)||2006|
|Not Applicable||Not Cited||Increased sodium channel immunofluorescence at myelinated and demyelinated sites following an inflammatory and partial axotomy lesion of the rat infraorbital nerve.||Henry MA,Freking AR,Johnson LR,Levinson SR||Pain (124:222)||2006|
|Not Applicable||Not Cited||Roles of endogenously synthesized sterols in the endocytic pathway.||Sugii S,Lin S,Ohgami N,Ohashi M,Chang CC,Chang TY||The Journal of biological chemistry (281:23191)||2006|
|Not Applicable||Not Cited||Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation.||Richardson RT,Alekseev OM,Grossman G,Widgren EE,Thresher R,Wagner EJ,Sullivan KD,Marzluff WF,O'Rand MG||The Journal of biological chemistry (281:21526)||2006|
|Not Applicable||Not Cited||Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2.||Andrews-Zwilling YS,Kawabe H,Reim K,Varoqueaux F,Brose N||The Journal of biological chemistry (281:19720)||2006|
|Not Applicable||Not Cited||Mutant nuclear lamin A leads to progressive alterations of epigenetic control in premature aging.||Shumaker DK,Dechat T,Kohlmaier A,Adam SA,Bozovsky MR,Erdos MR,Eriksson M,Goldman AE,Khuon S,Collins FS,Jenuwein T,Goldman RD||Proceedings of the National Academy of Sciences of the United States of America (103:8703)||2006|
|Not Applicable||Not Cited||Functional and selective RNA interference in developing axons and growth cones.||Hengst U,Cox LJ,Macosko EZ,Jaffrey SR||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:5727)||2006|
|Not Applicable||Not Cited||Attenuated spread of X-inactivation in an X;autosome translocation.||Popova BC,Tada T,Takagi N,Brockdorff N,Nesterova TB||Proceedings of the National Academy of Sciences of the United States of America (103:7706)||2006|
|Not Applicable||Not Cited||A role for ADP-ribosylation factor 6 in the processing of G-protein-coupled receptors.||Madziva MT,Birnbaumer M||The Journal of biological chemistry (281:12178)||2006|
|Not Applicable||Not Cited||Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle.||Hanft LM,Rybakova IN,Patel JR,Rafael-Fortney JA,Ervasti JM||Proceedings of the National Academy of Sciences of the United States of America (103:5385)||2006|
|Not Applicable||Not Cited||Novel role for RbAp48 in tissue-specific, estrogen deficiency-dependent apoptosis in the exocrine glands.||Ishimaru N,Arakaki R,Omotehara F,Yamada K,Mishima K,Saito I,Hayashi Y||Molecular and cellular biology (26:2924)||2006|
|Not Applicable||Not Cited||Chx10 is required to block photoreceptor differentiation but is dispensable for progenitor proliferation in the postnatal retina.||Livne-Bar I,Pacal M,Cheung MC,Hankin M,Trogadis J,Chen D,Dorval KM,Bremner R||Proceedings of the National Academy of Sciences of the United States of America (103:4988)||2006|
|Not Applicable||Not Cited||Form deprivation modulates retinal neurogenesis in primate experimental myopia.||Tkatchenko AV,Walsh PA,Tkatchenko TV,Gustincich S,Raviola E||Proceedings of the National Academy of Sciences of the United States of America (103:4681)||2006|
|Not Applicable||Not Cited||A Maurer's cleft-associated protein is essential for expression of the major malaria virulence antigen on the surface of infected red blood cells.||Cooke BM,Buckingham DW,Glenister FK,Fernandez KM,Bannister LH,Marti M,Mohandas N,Coppel RL||The Journal of cell biology (172:899)||2006|
|Not Applicable||Not Cited||Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.||Corrotte M,Chasserot-Golaz S,Huang P,Du G,Ktistakis NT,Frohman MA,Vitale N,Bader MF,Grant NJ||Traffic (Copenhagen, Denmark) (7:365)||2006|
|Not Applicable||Not Cited||Decreased renal organic anion secretion and plasma accumulation of endogenous organic anions in OAT1 knock-out mice.||Eraly SA,Vallon V,Vaughn DA,Gangoiti JA,Richter K,Nagle M,Monte JC,Rieg T,Truong DM,Long JM,Barshop BA,Kaler G,Nigam SK||The Journal of biological chemistry (281:5072)||2006|
|Not Applicable||Not Cited||The adult Drosophila posterior midgut is maintained by pluripotent stem cells.||Ohlstein B,Spradling A||Nature (439:470)||2006|
|Not Applicable||Not Cited||Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.||Goffin JM,Pittet P,Csucs G,Lussi JW,Meister JJ,Hinz B||The Journal of cell biology (172:259)||2006|
|Not Applicable||Not Cited||Protein fucosylation regulates synapsin Ia/Ib expression and neuronal morphology in primary hippocampal neurons.||Murrey HE,Gama CI,Kalovidouris SA,Luo WI,Driggers EM,Porton B,Hsieh-Wilson LC||Proceedings of the National Academy of Sciences of the United States of America (103:21)||2006|
|Not Applicable||Not Cited||Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves.||Amoh Y,Li L,Campillo R,Kawahara K,Katsuoka K,Penman S,Hoffman RM||Proceedings of the National Academy of Sciences of the United States of America (102:17734)||2005|
|Not Applicable||Not Cited||Extracellular application of nicotinic acid adenine dinucleotide phosphate induces Ca2+ signaling in astrocytes in situ.||Heidemann AC,Schipke CG,Kettenmann H||The Journal of biological chemistry (280:35630)||2005|
|Not Applicable||Not Cited||Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.||Hu T,Ramachandrarao SP,Siva S,Valancius C,Zhu Y,Mahadev K,Toh I,Goldstein BJ,Woolkalis M,Sharma K||American journal of physiology. Renal physiology (289:F816)||2005|
|Not Applicable||Not Cited||Centrosome localization determines neuronal polarity.||de Anda FC,Pollarolo G,Da Silva JS,Camoletto PG,Feiguin F,Dotti CG||Nature (436:704)||2005|
|Not Applicable||Not Cited||Src regulates distinct pathways for cell volume control through Vav and phospholipase Cgamma.||Barfod ET,Moore AL,Melnick RF,Lidofsky SD||The Journal of biological chemistry (280:25548)||2005|
|Not Applicable||Not Cited||Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.||Bates D,Kleckner N||Cell (121:899)||2005|
|Not Applicable||Not Cited||Early development of functional spatial maps in the zebrafish olfactory bulb.||Li J,Mack JA,Souren M,Yaksi E,Higashijima S,Mione M,Fetcho JR,Friedrich RW||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:5784)||2005|
|Not Applicable||Not Cited||Capturing protein interactions in the secretory pathway of living cells.||Nyfeler B,Michnick SW,Hauri HP||Proceedings of the National Academy of Sciences of the United States of America (102:6350)||2005|
|Not Applicable||Not Cited||Hypoxia-inducible factor-1-dependent overexpression of myeloid cell factor-1 protects hypoxic cells against tert-butyl hydroperoxide-induced apoptosis.||Piret JP,Minet E,Cosse JP,Ninane N,Debacq C,Raes M,Michiels C||The Journal of biological chemistry (280:9336)||2005|
|Not Applicable||Not Cited||Regulation of latent sensory hair cell precursors by glia in the zebrafish lateral line.||Grant KA,Raible DW,Piotrowski T||Neuron (45:69)||2005|
|Not Applicable||Not Cited||Gbetagamma activation of Src induces caveolae-mediated endocytosis in endothelial cells.||Shajahan AN,Tiruppathi C,Smrcka AV,Malik AB,Minshall RD||The Journal of biological chemistry (279:48055)||2004|
|Not Applicable||Not Cited||Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.||Ohka S,Matsuda N,Tohyama K,Oda T,Morikawa M,Kuge S,Nomoto A||Journal of virology (78:7186)||2004|
|Not Applicable||Not Cited||Distal renal tubular acidosis in mice that lack the forkhead transcription factor Foxi1.||Blomqvist SR,Vidarsson H,Fitzgerald S,Johansson BR,Ollerstam A,Brown R,Persson AE,Bergström G Gö,Enerbäck S||The Journal of clinical investigation (113:1560)||2004|
|Not Applicable||Not Cited||Specification of the retinal fate of mouse embryonic stem cells by ectopic expression of Rx/rax, a homeobox gene.||Tabata Y,Ouchi Y,Kamiya H,Manabe T,Arai K,Watanabe S||Molecular and cellular biology (24:4513)||2004|
|Not Applicable||Not Cited||Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells.||Fuja TJ,Schwartz PH,Darcy D,Bryant PJ||Journal of neuroscience research (75:782)||2004|
|Not Applicable||Not Cited||Distinctions in meiotic spindle structure and assembly during in vitro and in vivo maturation of mouse oocytes.||Sanfins A,Lee GY,Plancha CE,Overstrom EW,Albertini DF||Biology of reproduction (69:2059)||2003|
|Not Applicable||Not Cited||A calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes.||Li N,Sul JY,Haydon PG||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:10302)||2003|
|Not Applicable||Not Cited||Desmosomal proteins, including desmoglein 3, serve as novel negative markers for epidermal stem cell-containing population of keratinocytes.||Wan H,Stone MG,Simpson C,Reynolds LE,Marshall JF,Hart IR,Hodivala-Dilke KM,Eady RA||Journal of cell science (116:4239)||2003|
|Not Applicable||Not Cited||Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol.||Sugii S,Reid PC,Ohgami N,Du H,Chang TY||The Journal of biological chemistry (278:27180)||2003|
|Not Applicable||Not Cited||The transmembrane domain and PXXP motifs of ApoE receptor 2 exclude it from carrying out clathrin-mediated endocytosis.||Sun XM,Soutar AK||The Journal of biological chemistry (278:19926)||2003|
|Not Applicable||Not Cited||A novel nontruncating APOB gene mutation, R463W, causes familial hypobetalipoproteinemia.||Burnett JR,Shan J,Miskie BA,Whitfield AJ,Yuan J,Tran K,McKnight CJ,Hegele RA,Yao Z||The Journal of biological chemistry (278:13442)||2003|
|Not Applicable||Not Cited||Organization and dynamics of growing microtubule plus ends during early mitosis.||Piehl M,Cassimeris L||Molecular biology of the cell (14:916)||2003|
|Not Applicable||Not Cited||Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength.||Huen AC,Park JK,Godsel LM,Chen X,Bannon LJ,Amargo EV,Hudson TY,Mongiu AK,Leigh IM,Kelsell DP,Gumbiner BM,Green KJ||The Journal of cell biology (159:1005)||2002|
|Not Applicable||Not Cited||Oligomerization, F-actin interaction, and membrane association of the ubiquitous mammalian coronin 3 are mediated by its carboxyl terminus.||Spoerl Z,Stumpf M,Noegel AA,Hasse A||The Journal of biological chemistry (277:48858)||2002|
|Not Applicable||Not Cited||Tumor suppressor activity of AP2alpha mediated through a direct interaction with p53.||McPherson LA,Loktev AV,Weigel RJ||The Journal of biological chemistry (277:45028)||2002|
|Not Applicable||Not Cited||MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction.||Harada A,Teng J,Takei Y,Oguchi K,Hirokawa N||The Journal of cell biology (158:541)||2002|
|Not Applicable||Not Cited||A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport.||Strom M,Hume AN,Tarafder AK,Barkagianni E,Seabra MC||The Journal of biological chemistry (277:25423)||2002|
|Not Applicable||Not Cited||Generation of a complete thymic microenvironment by MTS24(+) thymic epithelial cells.||Gill J,Malin M,Holländer GA,Boyd R||Nature immunology (3:635)||2002|
|Not Applicable||Not Cited||Structure-function analysis of NADE: identification of regions that mediate nerve growth factor-induced apoptosis.||Mukai J,Shoji S,Kimura MT,Okubo S,Sano H,Suvanto P,Li Y,Irie S,Sato TA||The Journal of biological chemistry (277:13973)||2002|
|Not Applicable||Not Cited||Structural requirements for localization and activation of protein kinase C mu (PKC mu) at the Golgi compartment.||Hausser A,Link G,Bamberg L,Burzlaff A,Lutz S,Pfizenmaier K,Johannes FJ||The Journal of cell biology (156:65)||2002|
|Not Applicable||Not Cited||DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency.||O'Driscoll M,Cerosaletti KM,Girard PM,Dai Y,Stumm M,Kysela B,Hirsch B,Gennery A,Palmer SE,Seidel J,Gatti RA,Varon R,Oettinger MA,Neitzel H,Jeggo PA,Concannon P||Molecular cell (8:1175)||2001|
|Not Applicable||Not Cited||Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells.||Zhao X,Várnai P,Tuymetova G,Balla A,Tóth ZE,Oker-Blom C,Roder J,Jeromin A,Balla T||The Journal of biological chemistry (276:40183)||2001|
|Not Applicable||Not Cited||Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation.||Schubert LA,Jeffery E,Zhang Y,Ramsdell F,Ziegler SF||The Journal of biological chemistry (276:37672)||2001|
|Not Applicable||Not Cited||In vivo requirement of the alpha-syntrophin PDZ domain for the sarcolemmal localization of nNOS and aquaporin-4.||Adams ME,Mueller HA,Froehner SC||The Journal of cell biology (155:113)||2001|
|Not Applicable||Not Cited||The verprolin-like central (vc) region of Wiskott-Aldrich syndrome protein induces Arp2/3 complex-dependent actin nucleation.||Hüfner K,Higgs HN,Pollard TD,Jacobi C,Aepfelbacher M,Linder S||The Journal of biological chemistry (276:35761)||2001|
|Not Applicable||Not Cited||Transglutaminase 5 cross-links loricrin, involucrin, and small proline-rich proteins in vitro.||Candi E,Oddi S,Terrinoni A,Paradisi A,Ranalli M,Finazzi-Agró A,Melino G||The Journal of biological chemistry (276:35014)||2001|
|Not Applicable||Not Cited||Regulation of neuronal traits by a novel transcriptional complex.||Ballas N,Battaglioli E,Atouf F,Andres ME,Chenoweth J,Anderson ME,Burger C,Moniwa M,Davie JR,Bowers WJ,Federoff HJ,Rose DW,Rosenfeld MG,Brehm P,Mandel G||Neuron (31:353)||2001|
|Not Applicable||Not Cited||Characterization and expression of calpain 10. A novel ubiquitous calpain with nuclear localization.||Ma H,Fukiage C,Kim YH,Duncan MK,Reed NA,Shih M,Azuma M,Shearer TR||The Journal of biological chemistry (276:28525)||2001|
|Not Applicable||Not Cited||The C2A domain of double C2 protein gamma contains a functional nuclear localization signal.||Fukuda M,Saegusa C,Kanno E,Mikoshiba K||The Journal of biological chemistry (276:24441)||2001|
|Not Applicable||Not Cited||An immunohistochemical method for the detection of proteins in the vertebrate lens.||Reed NA,Oh DJ,Czymmek KJ,Duncan MK||Journal of immunological methods (253:243)||2001|
|Not Applicable||Not Cited||Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes: interference with early steps of viral replication.||Turelli P,Doucas V,Craig E,Mangeat B,Klages N,Evans R,Kalpana G,Trono D||Molecular cell (7:1245)||2001|
|Not Applicable||Not Cited||Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells.||Yoon KH,Yoon M,Moir RD,Khuon S,Flitney FW,Goldman RD||The Journal of cell biology (153:503)||2001|
|Not Applicable||Not Cited||Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin.||Kessels MM,Engqvist-Goldstein AE,Drubin DG,Qualmann B||The Journal of cell biology (153:351)||2001|
|Not Applicable||Not Cited||Murine cytomegalovirus M78 protein, a G protein-coupled receptor homologue, is a constituent of the virion and facilitates accumulation of immediate-early viral mRNA.||Oliveira SA,Shenk TE||Proceedings of the National Academy of Sciences of the United States of America (98:3237)||2001|
|Not Applicable||Not Cited||Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer.||Sato K,Sato M,Nakano A||The Journal of cell biology (152:935)||2001|
|Not Applicable||Not Cited||Rab27a regulates the peripheral distribution of melanosomes in melanocytes.||Hume AN,Collinson LM,Rapak A,Gomes AQ,Hopkins CR,Seabra MC||The Journal of cell biology (152:795)||2001|
|Not Applicable||Not Cited||Circadian activation of bullfrog retinal mitogen-activated protein kinase associates with oscillator function.||Harada Y,Sanada K,Fukada Y||The Journal of biological chemistry (275:37078)||2000|
|Not Applicable||Not Cited||A novel motor, KIF13A, transports mannose-6-phosphate receptor to plasma membrane through direct interaction with AP-1 complex.||Nakagawa T,Setou M,Seog D,Ogasawara K,Dohmae N,Takio K,Hirokawa N||Cell (103:569)||2000|
|Not Applicable||Not Cited||Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.||Waizenegger IC,Hauf S,Meinke A,Peters JM||Cell (103:399)||2000|
|Not Applicable||Not Cited||Endothelial cell-surface gp60 activates vesicle formation and trafficking via G(i)-coupled Src kinase signaling pathway.||Minshall RD,Tiruppathi C,Vogel SM,Niles WD,Gilchrist A,Hamm HE,Malik AB||The Journal of cell biology (150:1057)||2000|
|Not Applicable||Not Cited||M-ABC2, a new human mitochondrial ATP-binding cassette membrane protein.||Zhang F,Hogue DL,Liu L,Fisher CL,Hui D,Childs S,Ling V||FEBS letters (478:89)||2000|
|Not Applicable||Not Cited||Regulated expression of nullo is required for the formation of distinct apical and basal adherens junctions in the Drosophila blastoderm.||Hunter C,Wieschaus E||The Journal of cell biology (150:391)||2000|
|Not Applicable||Not Cited||Ectopic scute induces Drosophila ommatidia development without R8 founder photoreceptors.||Sun Y,Jan LY,Jan YN||Proceedings of the National Academy of Sciences of the United States of America (97:6815)||2000|
|Not Applicable||Not Cited||Sodium channel Na(v)1.6 is localized at nodes of ranvier, dendrites, and synapses.||Caldwell JH,Schaller KL,Lasher RS,Peles E,Levinson SR||Proceedings of the National Academy of Sciences of the United States of America (97:5616)||2000|
|Not Applicable||Not Cited||Multiple growth factor induction of a murine early response gene that complements a lethal defect in yeast ribosome biogenesis.||Nelson SA,Aris JP,Patel BK,LaRochelle WJ||The Journal of biological chemistry (275:13835)||2000|
|Not Applicable||Not Cited||Rab7: a key to lysosome biogenesis.||Bucci C,Thomsen P,Nicoziani P,McCarthy J,van Deurs B||Molecular biology of the cell (11:467)||2000|
|Not Applicable||Not Cited||Toxofilin, a novel actin-binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments.||Poupel O,Boleti H,Axisa S,Couture-Tosi E,Tardieux I||Molecular biology of the cell (11:355)||2000|
|Not Applicable||Not Cited||Complement activation occurs on subendothelial extracellular matrix in vitro and is initiated by retraction or removal of overlying endothelial cells.||Hindmarsh EJ,Marks RM||Journal of immunology (Baltimore, Md. : 1950) (160:6128)||1998|
|Not Applicable||Not Cited||Slow light and dark adaptation of horizontal cells in the Xenopus retina: a role for endogenous dopamine.||Witkovsky P,Shi XP||Visual neuroscience (5:405)||1990|
Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300.
A-11011 was used in immunohistochemistry - paraffin section to determine the role of lysine acetyltransferases p300 and CBP and how lens induction requires them and histone posttranslational modification and cell fate determination
|Wolf L,Harrison W,Huang J,Xie Q,Xiao N,Sun J,Kong L,Lachke SA,Kuracha MR,Govindarajan V,Brindle PK,Ashery-Padan R,Beebe DC,Overbeek PA,Cvekl A||Nucleic acids research (41:10199)||2013|
Tight junction proteins in human Schwann cell autotypic junctions.
A-11011 was used in immunohistochemistry - frozen section to study the expression of adherens junction and tight junction components in human peripheral nerve endoneurium
|Alanne MH,Pummi K,Heape AM,Grènman R,Peltonen J,Peltonen S||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (57:523)||2009|