|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 750|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Western Blot (WB)||0.04-0.2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Physical and functional association of lactate dehydrogenase (LDH) with skeletal muscle mitochondria.||Elustondo PA,White AE,Hughes ME,Brebner K,Pavlov E,Kane DA||The Journal of biological chemistry (288:25309)||2013|
|Not Applicable||Not Cited||Costimulatory protein 4IgB7H3 drives the malignant phenotype of glioblastoma by mediating immune escape and invasiveness.||Lemke D,Pfenning PN,Sahm F,Klein AC,Kempf T,Warnken U,Schnölzer M,Tudoran R,Weller M,Platten M,Wick W||Clinical cancer research : an official journal of the American Association for Cancer Research (18:105)||2012|
|Not Applicable||Not Cited||4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.||Baddeley D,Crossman D,Rossberger S,Cheyne JE,Montgomery JM,Jayasinghe ID,Cremer C,Cannell MB,Soeller C||PloS one (6:null)||2011|
|Not Applicable||Not Cited||Quantitative evaluation of signaling events in Drosophila S2 cells.||Bond D,Primrose DA,Foley E||Biological procedures online (10:20)||2008|
|Not Applicable||Not Cited||Self-renewing and differentiating properties of cortical neural stem cells are selectively regulated by basic fibroblast growth factor (FGF) signaling via specific FGF receptors.||Maric D,Fiorio Pla A,Chang YH,Barker JL||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:1836)||2007|
|Not Applicable||Not Cited||Activation of endogenous Cdc42 visualized in living cells.||Nalbant P,Hodgson L,Kraynov V,Toutchkine A,Hahn KM||Science (New York, N.Y.) (305:1615)||2004|