Four-color fluorescence in situ hybridization on a Drosophila embryo. A late blastoderm stage (nuclear cycle 14) embryo was probed with four different RNA probes. Blue: sog labeled with DNP, followed by a rabbit anti-dinitrophenyl-KLH IgG antibody (Prod # A6430) detected with an Alexa Fluor® 647 chicken anti-rabbit IgG antibody (Prod # A21443). Green: ind labeled with biotin, followed by streptavidin HRP and Alexa Fluor® 350 tyramide (TSA Kit #27, Prod # T20937). Red: msh labeled with digoxigenin followed by sheep anti-digoxigenin antibody detected with an Alexa Fluor® 488 donkey anti-sheep IgG antibody (Prod # A11015). Yellow: sna labeled with fluorescein followed by mouse anti-fluorescein antibody detected with an Alexa Fluor® 555 goat anti-mouse IgG antibody (Prod # A21424). Image contributed by Dave Kosman and Ethan Bier, University of California, San Diego.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 647|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human and mouse IgG prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This chicken anti-rabbit antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Flourescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel ß subunits.
A-21443 was used in immunocytochemistry to characterize multimeric cell-adhesive interactions of voltage-gated sodium channel beta subunits by structure-based site-directed photo-crosslinking analyses
|Shimizu H,Miyazaki H,Ohsawa N,Shoji S,Ishizuka-Katsura Y,Tosaki A,Oyama F,Terada T,Sakamoto K,Shirouzu M,Sekine S,Nukina N,Yokoyama S||Scientific reports (6:null)||2016|
|Not Applicable||Not Cited||Human HMGCS2 regulates mitochondrial fatty acid oxidation and FGF21 expression in HepG2 cell line.||Vilà-Brau A,De Sousa-Coelho AL,Mayordomo C,Haro D,Marrero PF||The Journal of biological chemistry (286:20423)||2011|
|Not Applicable||Not Cited||Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.||Yamakawa N,Uchida T,Matthay MA,Makita K||American journal of physiology. Lung cellular and molecular physiology (300:L516)||2011|
|Not Applicable||Not Cited||Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice.||Bem RA,Farnand AW,Wong V,Koski A,Rosenfeld ME,van Rooijen N,Frevert CW,Martin TR,Matute-Bello G||American journal of physiology. Lung cellular and molecular physiology (295:L314)||2008|
|Not Applicable||Not Cited||Human TOB, an antiproliferative transcription factor, is a poly(A)-binding protein-dependent positive regulator of cytoplasmic mRNA deadenylation.||Ezzeddine N,Chang TC,Zhu W,Yamashita A,Chen CY,Zhong Z,Yamashita Y,Zheng D,Shyu AB||Molecular and cellular biology (27:7791)||2007|
|Not Applicable||Not Cited||Stat5a inhibits IL-12-induced Th1 cell differentiation through the induction of suppressor of cytokine signaling 3 expression.||Takatori H,Nakajima H,Kagami S,Hirose K,Suto A,Suzuki K,Kubo M,Yoshimura A,Saito Y,Iwamoto I||Journal of immunology (Baltimore, Md. : 1950) (174:4105)||2005|
|Not Applicable||Not Cited||Multiplex detection of RNA expression in Drosophila embryos.||Kosman D,Mizutani CM,Lemons D,Cox WG,McGinnis W,Bier E||Science (New York, N.Y.) (305:null)||2004|