|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 405|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High-performance probes for light and electron microscopy.
A-31556 was used in immunohistochemistry to develop and characterize 'spaghetti monster' fluorescent proteins
|Viswanathan S,Williams ME,Bloss EB,Stasevich TJ,Speer CM,Nern A,Pfeiffer BD,Hooks BM,Li WP,English BP,Tian T,Henry GL,Macklin JJ,Patel R,Gerfen CR,Zhuang X,Wang Y,Rubin GM,Looger LL||Nature methods (12:568)||2015|
|Not Applicable||Not Cited||Regulation of type VI secretion system during Burkholderia pseudomallei infection.||Chen Y,Wong J,Sun GW,Liu Y,Tan GY,Gan YH||Infection and immunity (79:3064)||2011|
|Not Applicable||Not Cited||Oncogenic Src requires a wild-type counterpart to regulate invadopodia maturation.||Kelley LC,Ammer AG,Hayes KE,Martin KH,Machida K,Jia L,Mayer BJ,Weed SA||Journal of cell science (123:3923)||2010|
|Not Applicable||Not Cited||Glycogen synthase kinase 3beta missplicing contributes to leukemia stem cell generation.||Abrahamsson AE,Geron I,Gotlib J,Dao KH,Barroga CF,Newton IG,Giles FJ,Durocher J,Creusot RS,Karimi M,Jones C,Zehnder JL,Keating A,Negrin RS,Weissman IL,Jamieson CH||Proceedings of the National Academy of Sciences of the United States of America (106:3925)||2009|
|Not Applicable||Not Cited||EHD3 regulates early-endosome-to-Golgi transport and preserves Golgi morphology.||Naslavsky N,McKenzie J,Altan-Bonnet N,Sheff D,Caplan S||Journal of cell science (122:389)||2009|
|Not Applicable||Not Cited||Essential role of PDK1 in regulating endothelial cell migration.||Primo L,di Blasio L,Roca C,Droetto S,Piva R,Schaffhausen B,Bussolino F||The Journal of cell biology (176:1035)||2007|