Western blot analysis of Desmin was performed by loading 20 ug of human hepatic stellate cell lysate and 10ul of Molecular Weight Protein Ladder (Product # 26634) per well onto a Novex Bolt 4-12% Bis-Tris plus polyacrylamide gel (NW04125). Proteins were transferred to a PVDF membrane using iBlot 2 transfer device (Product #IB21001), and blocked with 5% non-fat dry milk in TBS+0.1%Tween-20 for one hour at room temperature. Desmin was detected at 53kDa using Desmin polyclonal antibody (Product # PA5-16705) at a dilution of 1:5000 at 4oC overnight on a rocking platform, followed by a goat anti-rabbit HRP conjugated antibody (Product # A24537) at a dilution of 1:7000 for one hour. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit(Product # WP20005).
|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.1% Kathon™ CG|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Cross Adsorption||Against bovine, human and mouse IgG and serum proteins|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.