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Specificity of our rabbit anti-nitrotyrosine polyclonal antibody (Cat. no. A21285) to nitrated proteins. Equal amounts of avidin (A887, lane 1) and CaptAvidin™ biotin-binding protein (Cat. no. C21385, lane 2) were run on an SDS-polyacrylamide gel (4–20%) and blotted onto a PVDF membrane. CaptAvidin™ biotin-binding protein, a derivative of avidin, has nitrated tyrosine residues in the biotin-binding site. Using the Pro-Q® Western Blot Stain Kit ##3 (Cat. no. P21864), nitrated proteins were identified with the rabbit anti-nitrotyrosine polyclonal antibody, in combination with an alkaline phosphatase conjugate of goat anti–rabbit IgG antibody (Cat. no. G21079) and the red-fluorescent substrate, DDAO phosphate (Cat. no. D6487).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||50mM tris, pH 8.0, with 30mM trehalose, 0.1M NaCl|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:500 to 1:2,000|
|Immunohistochemistry (IHC)||1:500 to 1:2,000|
|Western Blot (WB)||1:500 to 1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 16 publications below|
The specific activity of the AP conjugates is approximately 150–350 units/mg. One unit is defined as the amount of enzymatic activity required to hydrolyze 1.0 micromole of p nitrophenyl phosphate to p-nitrophenol and inorganic phosphate in 60 seconds at 21°C, pH 10.
Reconstituted using 0.5 mL of deionized water to make a 2 mg/mL stock solution.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Modulation of Leydig cell function by cyclic nucleotide phosphodiesterase 8A.||Vasta V,Shimizu-Albergine M,Beavo JA||Proceedings of the National Academy of Sciences of the United States of America (103:19925)||2006|
|Not Applicable||Not Cited||Humoral immune response to fibrillar beta-amyloid peptide.||Miller DL,Currie JR,Mehta PD,Potempska A,Hwang YW,Wegiel J||Biochemistry (42:11682)||2003|
|Not Applicable||Not Cited||MAHRP-1, a novel Plasmodium falciparum histidine-rich protein, binds ferriprotoporphyrin IX and localizes to the Maurer's clefts.||Spycher C,Klonis N,Spielmann T,Kump E,Steiger S,Tilley L,Beck HP||The Journal of biological chemistry (278:35373)||2003|
|Not Applicable||Not Cited||ClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl- channels and volume regulation in HeLa cells and Xenopus laevis oocytes.||Hermoso M,Satterwhite CM,Andrade YN,Hidalgo J,Wilson SM,Horowitz B,Hume JR||The Journal of biological chemistry (277:40066)||2002|
|Not Applicable||Not Cited||Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.||Conner SD,Schmid SL||The Journal of cell biology (156:921)||2002|
|Not Applicable||Not Cited||Structural requirements for localization and activation of protein kinase C mu (PKC mu) at the Golgi compartment.||Hausser A,Link G,Bamberg L,Burzlaff A,Lutz S,Pfizenmaier K,Johannes FJ||The Journal of cell biology (156:65)||2002|
|Not Applicable||Not Cited||Suppression of tumor-related glycosylation of cell surface receptors by the 16-kDa membrane subunit of vacuolar H+-ATPase.||Skinner MA,Wildeman AG||The Journal of biological chemistry (276:48451)||2001|
|Not Applicable||Not Cited||Synchronized activation and refolding of influenza hemagglutinin in multimeric fusion machines.||Markovic I,Leikina E,Zhukovsky M,Zimmerberg J,Chernomordik LV||The Journal of cell biology (155:833)||2001|
|Not Applicable||Not Cited||Munc18-1 promotes large dense-core vesicle docking.||Voets T,Toonen RF,Brian EC,de Wit H,Moser T,Rettig J,Südhof TC,Neher E,Verhage M||Neuron (31:581)||2001|
|Not Applicable||Not Cited||Differential assembly of rat purinergic P2X7 receptor in immune cells of the brain and periphery.||Kim M,Spelta V,Sim J,North RA,Surprenant A||The Journal of biological chemistry (276:23262)||2001|
|Not Applicable||Not Cited||Functional reconstitution and characterization of recombinant human alpha 1-glycine receptors.||Cascio M,Shenkel S,Grodzicki RL,Sigworth FJ,Fox RO||The Journal of biological chemistry (276:20981)||2001|
|Not Applicable||Not Cited||Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo.||Bronk P,Wenniger JJ,Dawson-Scully K,Guo X,Hong S,Atwood HL,Zinsmaier KE||Neuron (30:475)||2001|
|Not Applicable||Not Cited||Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth.||Calero M,Whittaker GR,Collins RN||The Journal of biological chemistry (276:12100)||2001|
|Not Applicable||Not Cited||Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in hepatocyte nucleus.||Khoo KM,Han MK,Park JB,Chae SW,Kim UH,Lee HC,Bay BH,Chang CF||The Journal of biological chemistry (275:24807)||2000|
|Not Applicable||Not Cited||PH-20 but not acrosin is involved in sperm penetration of the macaque zona pellucida.||Yudin AI,Vandevoort CA,Li MW,Overstreet JW||Molecular reproduction and development (53:350)||1999|
|Not Applicable||Not Cited||Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases.||Cooper AA,Stevens TH||The Journal of cell biology (133:529)||1996|