A human tonsil section visualized with fluorescent Qdot® nanocrystal conjugates. Ki67 in the nuclei of proliferating B-cells was labeled with a rabbit anti-Ki67 primary antibody and visualized using red-fluorescent Qdot® 585 goat F(ab')2 anti-rabbit IgG (Q11411MP). Keratin in the peripheral stromal cells was labeled with a mouse anti-pan-keratin monoclonal antibody and visualized using a biotin-goat anti-mouse IgG followed by a green-fluorescent Qdot® 525 streptavidin conjugate (Q10141MP). Nuclei were stained with blue-fluorescent Hoechst 33342 (H1399, H3570, H21492).
|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.