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HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-B-catenin antibody (712700) for 60 min at room temperature followed by three washes with dPBS. Cells were then incubated with a 1:1000 dilution of goat anti-rabbit Qdot® 655 secondary antibody (Q11421MP) for 60 min, followed by three washes with dPBS. Cells were labeled with NucBlue® Live cell stain (R37605) and ActinGreen™ 488 ReadyProbes® reagent (R37110) according to protocol. Coverslips were then mounted using ProLong® Gold Antifade Reagent (P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X Coverslip corrected objective (AMEP4699).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||0.05M borate, pH 8.3, with 1M betaine|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 9 publications below|
Qdot nanocrystals are composed of semi-conductor material to generate a fluorescent particle which is exceptionally bright and does not photobleach. Qdot nanocrystals paired with the correct optical filters are as much as 50 times brighter than traditional organic dyes.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots.||Liu J,Lau SK,Varma VA,Moffitt RA,Caldwell M,Liu T,Young AN,Petros JA,Osunkoya AO,Krogstad T,Leyland-Jones B,Wang MD,Nie S||ACS nano (4:2755)||2010|
|Not Applicable||Not Cited||Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates.||Kingeter LM,Schaefer BC||BMC biotechnology (9:null)||2009|
|Not Applicable||Not Cited||Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity.||Ehlers MD,Heine M,Groc L,Lee MC,Choquet D||Neuron (54:447)||2007|
|Not Applicable||Not Cited||Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms.||Chalmers NI,Palmer RJ,Du-Thumm L,Sullivan R,Shi W,Kolenbrander PE||Applied and environmental microbiology (73:630)||2007|
|Not Applicable||Not Cited||NMDA receptor surface mobility depends on NR2A-2B subunits.||Groc L,Heine M,Cousins SL,Stephenson FA,Lounis B,Cognet L,Choquet D||Proceedings of the National Academy of Sciences of the United States of America (103:18769)||2006|
|Not Applicable||Not Cited||Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.||Ferrara DE,Weiss D,Carnell PH,Vito RP,Vega D,Gao X,Nie S,Taylor WR||American journal of physiology. Regulatory, integrative and comparative physiology (290:R114)||2006|
|Not Applicable||Not Cited||Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots.||Giepmans BN,Deerinck TJ,Smarr BL,Jones YZ,Ellisman MH||Nature methods (2:743)||2005|
|Not Applicable||Not Cited||Three-dimensional imaging of the intracellular localization of growth hormone and prolactin and their mRNA using nanocrystal (Quantum dot) and confocal laser scanning microscopy techniques.||Matsuno A,Itoh J,Takekoshi S,Nagashima T,Osamura RY||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:833)||2005|
|Not Applicable||Not Cited||Detection of Mycobacterium bovis Bacillus Calmette-Guerin using quantum dot immuno-conjugates.||Otsuka Y,Hanaki K,Zhao J,Ohtsuki R,Toyooka K,Yoshikura H,Kuratsuji T,Yamamoto K,Kirikae T||Japanese journal of infectious diseases (57:183)||2004|