|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Cross Adsorption||Against human and rat serum proteins prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:500 to 1:2,000|
|Immunohistochemistry (IHC)||1:500 to 1:2,000|
|Western Blot (WB)||1:500 to 1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Storage and reconstitution: when stored desiccated at < -20°C, the lyophilized powder is stable for at least one year. These products may be reconstituted in 1 mL PBS, pH 7.2, to yield a 1 mg/mL stock solution.
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Horseradish peroxidase: a modern view of a classic enzyme.||Veitch NC||Phytochemistry (65:249)||2004|