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|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human and rat serum proteins prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 9 publications below|
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Cerebral small-vessel disease protein HTRA1 controls the amount of TGF-β1 via cleavage of proTGF-β1.||Shiga A,Nozaki H,Yokoseki A,Nihonmatsu M,Kawata H,Kato T,Koyama A,Arima K,Ikeda M,Katada S,Toyoshima Y,Takahashi H,Tanaka A,Nakano I,Ikeuchi T,Nishizawa M,Onodera O||Human molecular genetics (20:1800)||2011|
|Not Applicable||Not Cited||Gamma-secretase complexes regulate the responses of human pancreatic ductal adenocarcinoma cells to taxanes.||Tasaka T,Akiyoshi T,Yamaguchi K,Tanaka M,Onishi H,Katano M||Anticancer research (30:4999)||2010|
|Not Applicable||Not Cited||Knockdown of mu-calpain in Fanconi anemia, FA-A, cells by siRNA restores alphaII spectrin levels and corrects chromosomal instability and defective DNA interstrand cross-link repair.||Zhang P,Sridharan D,Lambert MW||Biochemistry (49:5570)||2010|
|Not Applicable||Not Cited||Lysophosphatidylcholine increases neutrophil bactericidal activity by enhancement of azurophil granule-phagosome fusion via glycine.GlyR alpha 2/TRPM2/p38 MAPK signaling.||Hong CW,Kim TK,Ham HY,Nam JS,Kim YH,Zheng H,Pang B,Min TK,Jung JS,Lee SN,Cho HJ,Kim EJ,Hong IH,Kang TC,Lee J,Oh SB,Jung SJ,Kim SJ,Song DK||Journal of immunology (Baltimore, Md. : 1950) (184:4401)||2010|
|Not Applicable||Not Cited||RAGE-mediated signaling contributes to intraneuronal transport of amyloid-beta and neuronal dysfunction.||Takuma K,Fang F,Zhang W,Yan S,Fukuzaki E,Du H,Sosunov A,McKhann G,Funatsu Y,Nakamichi N,Nagai T,Mizoguchi H,Ibi D,Hori O,Ogawa S,Stern DM,Yamada K,Yan SS||Proceedings of the National Academy of Sciences of the United States of America (106:20021)||2009|
|Not Applicable||Not Cited||Satellite cell-mediated angiogenesis in vitro coincides with a functional hypoxia-inducible factor pathway.||Rhoads RP,Johnson RM,Rathbone CR,Liu X,Temm-Grove C,Sheehan SM,Hoying JB,Allen RE||American journal of physiology. Cell physiology (296:C1321)||2009|
|Not Applicable||Not Cited||Steroidogenic factor 1/adrenal 4 binding protein transforms human bone marrow mesenchymal cells into steroidogenic cells.||Tanaka T,Gondo S,Okabe T,Ohe K,Shirohzu H,Morinaga H,Nomura M,Tani K,Takayanagi R,Nawata H,Yanase T||Journal of molecular endocrinology (39:343)||2007|
|Not Applicable||Not Cited||Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase.||Woronowicz A,Amith SR,De Vusser K,Laroy W,Contreras R,Basta S,Szewczuk MR||Glycobiology (17:10)||2007|
|Not Applicable||Not Cited||Platelet-activating factor receptor and innate immunity: uptake of gram-positive bacterial cell wall into host cells and cell-specific pathophysiology.||Fillon S,Soulis K,Rajasekaran S,Benedict-Hamilton H,Radin JN,Orihuela CJ,El Kasmi KC,Murti G,Kaushal D,Gaber MW,Weber JR,Murray PJ,Tuomanen EI||Journal of immunology (Baltimore, Md. : 1950) (177:6182)||2006|