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|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-774 detects rabphilin 3A from rat tissues.
PA1-774 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~75 kDa protein representing rabphilin 3A from rat brain whole protein extract.
PA1-774 immunogen is a synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A. This sequence is completely conserved between rat and mouse rabphilin 3A. PA1-774 immunizing peptide (Cat. # PEP-102) is available for use in neutralization and control experiments.
Rabphilin 3A, a target protein for the small GTPase binding protein rab 3A, plays a role in regulated secretion, particularly in neurotransmitter release. Double labeling of rab 3A and rabphilin 3A shows the two proteins to be co-localized in nerve terminals of the spinal cord and motor endplates. Rabphilin binds selectively to the GTP bound form of rab 3A as well as of rab 3c. Studies suggest that rabphilin 3A phosphorylation by CaM kinase II modulates neurotransmitter release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
exophilin-1; rabphilin 3A homolog; rabphilin-3A; Rph3a