|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||N-terminus fusion protein of the C-terminal fragment (residues 544-631) of the Scc1 subunit of human cohesin.|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, two bands are seen: the full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
Suggested positive control: antigen standard for RAD21 (transient overexpression lysate), Hela whole cell extract.
The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit, allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.