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|Tested species reactivity||Human, Mouse, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Rad6.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
Immunohistochemistry has been used succesfully with methylmethacrylate sections.
This antibody is predicted to react with rabbit, chicken, cow, Saccharomyces cerevisiae, Xenopus laevis and fruit fly (Drosophila melanogaster) based on sequence homology.
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
E2 ubiquitin-conjugating enzyme A; HHR6A, MRXS30, MRXSN, RAD6A, UBC2, HR6A; RAD6 homolog A; ubiquitin carrier protein A; ubiquitin conjugating enzyme E2A; ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2A, RAD6 homolog; ubiquitin-conjugating enzyme HR6A; ubiquitin-protein ligase A
HHR6A; HR6A; Mhr6a; mp:zf637-2-000771; MRXS30; MRXSN; RAD6A; si:bz46j2.4; UBC2; UBE2A; wu:fa01h11