|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide corresponding to the sequence of human RagA protein.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/ml BSA|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The mTORC1 kinase complex is a critical regulator of cell growth. Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways. Four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex. These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.