Immunofluorescent analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody (Product # MA1-147) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant full-length human Rap1|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of MA1-147 detects an ~21kDa protein. In IHC (paraffin) applications, this antibody has only shown reactivity with human samples.
Rap1 is part of the Ras family of GTPases and is encoded by Rap1a and Rap1b. Rap1 cycles between two conformational states depending on whether GDP bound (active form) or GTP bound (inactive form) and primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion and tumor invasion. Rap1 may also regulate proliferation, differentiation and survival through multiple downstream effectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.