|Tested species reactivity||Amphibian, Chicken, Fish, Mouse, Rat|
|Published species reactivity||Rat, Amphibian, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Whole purified rapsyn from Torpedo californica electric organ postsynaptic membrane.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||2 ug/ml|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-746 detects rapysn from mouse, rat, chicken, amphibian and fish samples.
MA1-746 has been used in immunochemistry procedures using cryosections, immunocytochemistry, immunofluorescence and Western Blot procedures. By Western blot, this antibody detects a 48 kDa protein representing rapsyn from Torpedo californica electrocyte cell extracts.
The MA1-746 immunogen is whole purified rapsyn from Torpedo californica electric organ postsynaptic membrane.
Nicotinic acetylcholine receptors (AChRs) are organized into high-density postsynaptic clusters at the motor endplate that are critical for efficient synaptic transmission. The organization of AChR and quote;s is carried out by the 43 kDa cytoplasmic protein rapsyn. Studies have indicated that mutations which impair the activity of rapsyn can lead to the formation of congenital myasthenic syndrome (CMS). CMS is characterized by poor development of the postsynaptic region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The dystroglycan complex is necessary for stabilization of acetylcholine receptor clusters at neuromuscular junctions and formation of the synaptic basement membrane.
MA1-746 was used in immunocytochemistry to investigate the functional properties of dystroglycans in acetylcholine receptors clustering and the assembly of basement membrane
|Jacobson C,Côté PD,Rossi SG,Rotundo RL,Carbonetto S||The Journal of cell biology (152:435)||2001|
A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation.
MA1-746 was used in immunocytochemistry to investigate the regulation of AChR cluster dispersal and formation by PTPase
|Dai Z,Peng HB||The Journal of cell biology (141:1613)||1998|
Rapsyn clusters neuronal acetylcholine receptors but is inessential for formation of an interneuronal cholinergic synapse.
MA1-746 was used in immunohistochemistry to investigate the possible regulation of acetylcholine receptor clustering by rapsyn at interneuronal synapses
|Feng G,Steinbach JH,Sanes JR||The Journal of neuroscience : the official journal of the Society for Neuroscience (18:4166)||1998|
Neural agrin induces ectopic postsynaptic specializations in innervated muscle fibers.
MA1-746 was used in immunohistochemistry to investigate the effect of neural agrin on the expression of postsynaptic protein components
|Meier T,Hauser DM,Chiquet M,Landmann L,Ruegg MA,Brenner HR||The Journal of neuroscience : the official journal of the Society for Neuroscience (17:6534)||1997|
Evidence for in situ and in vitro association between beta-dystroglycan and the subsynaptic 43K rapsyn protein. Consequence for acetylcholine receptor clustering at the synapse.
MA1-746 was used in western blot to investigate the interaction between beta-dystroglycan and rapsyn
|Cartaud A,Coutant S,Petrucci TC,Cartaud J||The Journal of biological chemistry (273:11321)||1998|