|Tested species reactivity||Bovine, Hamster, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Rabbit, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat DNA polymerase beta protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13896 targets Rat DNA Polymerase beta in IP and WB applications and shows reactivity with Bovine, Hamster, Human, mouse, Rat, and Xenopus laevis samples.
The MA5-13896 immunogen is rat DNA polymerase beta protein.
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA polymerase-ß mediates the neurogenic effect of ß-amyloid protein in cultured subventricular zone neurospheres.
MA5-13896 was used in western blot to study the neurogenic effects of Abeta (1-42) on neurospheres from the murine subventricular zone and the key role of DNA polymerase-beta
|Calafiore M,Copani A,Deng W||Journal of neuroscience research (90:559)||2012|
Oxidative stress alters base excision repair pathway and increases apoptotic response in apurinic/apyrimidinic endonuclease 1/redox factor-1 haploinsufficient mice.
MA5-13896 was used in western blot to study the role of oxidative stress in the base excision repair pathway and apoptosis
|Unnikrishnan A,Raffoul JJ,Patel HV,Prychitko TM,Anyangwe N,Meira LB,Friedberg EC,Cabelof DC,Heydari AR||Free radical biology and medicine (46:1488)||2009|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-13896 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
Possible role of DNA polymerase beta in protecting human bronchial epithelial cells against cytotoxicity of hydroquinone.
MA5-13896 was used in western blot to study the role of DNA polymerase beta in protecting human bronchial epithelial cells against hydroquinone cytotoxicity
|Hu DL,Tang HW,Liang HR,Tang DS,Liu YM,Ji WD,Yuan JH,He Y,Zhu ZY,Yang JP,Fang DK,Sha Y,Tu XZ,Zhuang ZX||Biomedical and environmental sciences : BES (20:171)||2007|
Methylation of DNA polymerase beta by protein arginine methyltransferase 1 regulates its binding to proliferating cell nuclear antigen.
MA5-13896 was used in western blot to study the role of methylation of DNA polymerase beta in regulating its binding to proliferating cell nuclear antigen
|El-Andaloussi N,Valovka T,Toueille M,Hassa PO,Gehrig P,Covic M,Hübscher U,Hottiger MO||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (21:26)||2007|
Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions.
MA5-13896 was used in western blot to study connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions
|Valiunas V,Polosina YY,Miller H,Potapova IA,Valiuniene L,Doronin S,Mathias RT,Robinson RB,Rosen MR,Cohen IS,Brink PR||The Journal of physiology (568:459)||2005|
The overexpression of specialized DNA polymerases in cancer.
MA5-13896 was used in western blot to study the expression of specialized DNA polymerases in cancer
|Albertella MR,Lau A,O'Connor MJ||DNA repair (4:583)||2005|
Imbalanced base excision repair in response to folate deficiency is accelerated by polymerase beta haploinsufficiency.
MA5-13896 was used in western blot to study the interaction of folate deficiency and beta-pol haploinsufficiency in DNA damage repair
|Cabelof DC,Raffoul JJ,Nakamura J,Kapoor D,Abdalla H,Heydari AR||The Journal of biological chemistry (279:36504)||2004|
Apurinic/apyrimidinic endonuclease (APE/REF-1) haploinsufficient mice display tissue-specific differences in DNA polymerase beta-dependent base excision repair.
MA5-13896 was used in western blot to study tissue-specific differences in DNA polymerase beta-dependent base excision repair in APE/REF-1 haploinsufficient mice
|Raffoul JJ,Cabelof DC,Nakamura J,Meira LB,Friedberg EC,Heydari AR||The Journal of biological chemistry (279:18425)||2004|
X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance.
MA5-13896 was used in western blot to investigate the influence of XRCC1 on camptothecin resistance
|Park SY,Lam W,Cheng YC||Cancer research (62:459)||2002|
Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering.
MA5-13896 was used in western blot to study the effects of dietary lipid levels on DNA damage and DNA repair pathways in experimentally induced atherosclerotic plaques
|Martinet W,Knaapen MW,De Meyer GR,Herman AG,Kockx MM||Circulation research (88:733)||2001|
A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment.
MA5-13896 was used in ChIP assay to study the role of a novel XRCC1-containing complex on cellular survival of methyl methanesulfonate treatment
|Luo H,Chan DW,Yang T,Rodriguez M,Chen BP,Leng M,Mu JJ,Chen D,Songyang Z,Wang Y,Qin J||Molecular and cellular biology (24:8356)||2004|