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|Tested species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat IgE myeloma proteins: IR162, IR1016, IR2 and IR410.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µg/ml (50 µl for 2x10^7 cells in 100 µl)|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SA1-25261 detects rat IgE. SA1-25261 is specific for the epsilon chain of rat immunoglobulin IgE and does not cross react with other classs of rat immunoglobulin.
SA1-25261 has been successfully used in FACS applications. Use 50 µl of the suggested working dilution to label 2x10^7 cells in 100 µl.
The SA1-25261 immunogen is rat IgE myeloma proteins: IR162, IR1016, IR2 and IR410.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Thermo Scientific Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.