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|Tested species reactivity||Rat|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred, store in dark|
|Cross Adsorption||Against mouse IgG|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-33282 targets Rat IgG in FACS applications. Cross-reactivity with mouse IgG has been reduced by adsorption.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Thermo Scientific Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.