|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS, pH 7.8, with sucrose|
|Contains||0.08% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against guinea pig, hamster, bovine, cat, chicken, dog, goat, horse, human, mouse, Rhesus monkey, sheep, and swine IgG, as well as bovine IgM, dog IgM, goat IgM,human IgM, mouse IgM, rat IgM and sheep|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 31219 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31219 reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody cross-reacts with hamster IgG (3%), guinea pig IgG (1%) and it has minimum cross-reactivity with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.5 ml of distilled water (1.0 mg/ml after restoration).
Thermo Scientific Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.