Immunofluorescence analysis of Goat anti-Rat IgG (H+L) Secondary Antibody, Cy3 was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/ml Rat primary antibody for 3 hours at room temperature. Goat anti-Rat IgG (H+L) Secondary Antibody, Cy3 (Product # A10522) was used at a concentration of 2µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:500|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods.
A10522 was used in immunohistochemistry - paraffin section to study the architecture of the mouse mammary gland using high-resolution 3D imaging
|Lloyd-Lewis B,Davis FM,Harris OB,Hitchcock JR,Lourenco FC,Pasche M,Watson CJ||Breast cancer research : BCR (18:null)||2016|
Single-cell lineage tracing in the mammary gland reveals stochastic clonal dispersion of stem/progenitor cell progeny.
A10522 was used in immunohistochemistry - paraffin section to examine the differentiation potential of adult mammary stem cells
|Davis FM,Lloyd-Lewis B,Harris OB,Kozar S,Winton DJ,Muresan L,Watson CJ||Nature communications (7:null)||2016|
Survival Motor Neuron (SMN) protein is required for normal mouse liver development.
A10522 was used in immunohistochemistry - frozen section to provide evidence that the liver is an important therapeutic target for spinal muscular atrophy
|Szunyogova E,Zhou H,Maxwell GK,Powis RA,Francesco M,Gillingwater TH,Parson SH||Scientific reports (6:null)||2016|
Gq signaling causes glomerular injury by activating TRPC6.
A10522 was used in immunohistochemistry - frozen section to develop and characterize a mouse model of glomerular disease
|Wang L,Jirka G,Rosenberg PB,Buckley AF,Gomez JA,Fields TA,Winn MP,Spurney RF||The Journal of clinical investigation (125:1913)||2015|
|Not Applicable||Not Cited||
Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland.
A10522 was used in immunohistochemistry - frozen section to use a knockout model to determine the role of Prom1 in the mammary gland
|Anderson LH,Boulanger CA,Smith GH,Carmeliet P,Watson CJ||Developmental dynamics : an official publication of the American Association of Anatomists (240:674)||2011|
Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist.
A10522 was used in western blot to test if targeting tropomysin receptor kinase B reduces radiation-induced cognitive deficits
|Yang P,Leu D,Ye K,Srinivasan C,Fike JR,Huang TT||Experimental neurology (279:178)||2016|
Identified Serotonin-Releasing Neurons Induce Behavioral Quiescence and Suppress Mating in Drosophila.
A10522 was used in immunohistochemistry - free floating to study factors that regulate arousal negatively and induce states of quiescence using Drosophila
|Pooryasin A,Fiala A||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:12792)||2015|