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|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Oregon Green® 488|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Analysis of mid1p, a protein required for placement of the cell division site, reveals a link between the nucleus and the cell surface in fission yeast.||Paoletti A,Chang F||Molecular biology of the cell (11:2757)||2000|
|Not Applicable||Not Cited||Normal fertilization occurs with eggs lacking the integrin alpha6beta1 and is CD9-dependent.||Miller BJ,Georges-Labouesse E,Primakoff P,Myles DG||The Journal of cell biology (149:1289)||2000|
|Not Applicable||Not Cited||Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin.||Flory MR,Moser MJ,Monnat RJ,Davis TN||Proceedings of the National Academy of Sciences of the United States of America (97:5919)||2000|
|Not Applicable||Not Cited||Beta(2)-adrenergic receptor down-regulation. Evidence for a pathway that does not require endocytosis.||Jockers R,Angers S,Da Silva A,Benaroch P,Strosberg AD,Bouvier M,Marullo S||The Journal of biological chemistry (274:28900)||1999|