Description: The monoclonal rat IgM, kappa is useful as an isotype control immunoglobulin.
Applications Reported: Rat IgM Isotype Control Functional Grade Purified has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgM Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Immunoglobulin M is the third most common serum Ig and can exist as pentamer where all heavy chains are identical and all light chains are identical or as a monomer (e.g. found on B lymphocytes as B - cell receptors). The large pentameric structure allows for building of bridges between encountered epitopes on molecules that are too distant as to be connected by smaller IgG antibodies. IgM is built as the first antibody during an immune response and is responsible for agglutination and cytolytic reactions since its pentameric structure gives it 10 free antigen binding sites in theory and it posseses a high avidity. Due to conformational constraints among the 10 Fab portions, IgM only has a valence of 5. Additionally, IgM is not as versatile as IgG, but it is of vital importance in complement activation and agglutination. IgM is predominantly found in the lymph fluid and the blood and is a very effective neutralizing agent in the early stages of disease. Elevated levels can be a sign of recent infection or exposure to antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.