BALB/c splenocytes were double-stained with lambda rat anti-mouse monoclonal antibody biotin conjugate (1 µg/10<sup>6</sup> cells) and a rat anti-mouse CD19-PE conjugate. Small lymphocytes were then gated and analyzed using a flow cytometer.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2b, kappa|
|Immunogen||mouse lambda chain|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Target||Lambda light chain|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
Antibody to the lambda light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Continued differentiation during B lymphopoiesis requires signals in addition to cell survival.
A15380 was used in flow cytometry to study the contribution of Ig heavy chain-containing receptors on B cell development.
|Tarlinton DM,Corcoran LM,Strasser A||International immunology (9:1481)||1997|