|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay-dependent|
|Immunocytochemistry (ICC)||0.2-1 ug/ml|
|Immunofluorescence (IF)||0.2-1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Immunoprecipitation (IP)||0.2-1 µg/ml|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23277 detects RbAp46/48 in Human samples.
MA1-23277 has been successfully used in Immunofluorescence, immunohistochemistry (paraffin) Immunoprecipitation and Western Blot procedures. Predicted molecular weight is 47.6kDa.
MA1-23277 immunogen corresponds to the complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.
MA1-23277 was used in RNA immunoprecipitation to study the Alu elements in ANRIL non-coding RNA at chromosome 9p21 can modulate atherogenic cell function through trans-regulation of gene networks
|Holdt LM,Hoffmann S,Sass K,Langenberger D,Scholz M,Krohn K,Finstermeier K,Stahringer A,Wilfert W,Beutner F,Gielen S,Schuler G,Gäbel G,Bergert H,Bechmann I,Stadler PF,Thiery J,Teupser D||PLoS genetics (9:null)||2013|
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
MA1-23277 was used in western blot to characterize RbAp46 and study its functional properties
|Qian YW,Lee EY||The Journal of biological chemistry (270:25507)||1995|