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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay-dependent|
|Immunocytochemistry (ICC)||0.2-1 ug/ml|
|Immunofluorescence (IF)||0.2-1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Immunoprecipitation (IP)||0.2-1 µg/ml|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-23277 detects RbAp46/48 in Human samples.
MA1-23277 has been successfully used in Immunofluorescence, immunohistochemistry (paraffin) Immunoprecipitation and Western Blot procedures. Predicted molecular weight is 47.6kDa.
MA1-23277 immunogen corresponds to the complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
MA1-23277 was used in western blot to characterize RbAp46 and study its functional properties
|Qian YW,Lee EY||The Journal of biological chemistry (270:25507)||1995|
CAF-1 p48 subunit; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; G1/S transition control protein-binding protein; G1/S transition control protein-binding protein RbAp46; histone acetyltransferase type B subunit 2; histone-binding protein RBBP4; histone-binding protein RBBP7; MSI1 protein homolog; nucleosome-remodeling factor subunit RBAP46; nucleosome-remodeling factor subunit RBAP48; NURF55; RBAP48; RBBP-4; RBBP-7; retinoblastoma binding protein 4; retinoblastoma binding protein 7; retinoblastoma-binding protein 4; retinoblastoma-binding protein 7; retinoblastoma-binding protein p46; retinoblastoma-binding protein p48; retinoblastoma-binding protein RbAp46
AA409861; AI173248; AU019541; BB114024; lin-53; mRbAp46; mRbAp48; NURF55; RBAP46; RBAP48; RBBP4; RBBP7