|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Functional Assay (FN)||Assay-dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-23273 detects RbAp48 in Human and mouse samples.
MA1-23273 has been successfully used in ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western Blot procedures. Positive control: MOLT4, HeLa, LB22. For ICC a permeabilization step is recommended; use at a dilution of 1:500-1:1000 in PBST containing 2%BSA and 10% normal serum at 4°C overnight.
MA1-23273 immunogen corresponds to The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
MA1-23273 was used in western blot to characterize RbAp46 and study its functional properties
|Qian YW,Lee EY||The Journal of biological chemistry (270:25507)||1995|