Invitrogen

RbAp48 Recombinant Rabbit Monoclonal Antibody (JU37-47)

Product Image Gallery

RbAp48 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of RbAP48 in SH-SY5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RbAP48 monoclonal antibody (Product # MA5-34613), followed by DAPI (blue).

RbAp48 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RbAP48 in paraffin-embedded human lung cancer tissue. Samples were incubated with RbAP48 monoclonal antibody (Product # MA5-34613), and followed by hematoxylin.

RbAp48 Antibody in Western Blot (WB) — Western blot analysis of RbAP48 in Lane 1: NIH-3T3, Lane 2: HeLa, Lane 3: Rat brain, Lane 4: Mouse testis. Samples were incubated with RbAP48 monoclonal antibody (Product # MA5-34613), at a dilution of 1:500.

RbAp48 Antibody in Flow Cytometry (Flow) — Flow cytometry of RbAP48 in HeLa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with RbAP48 monoclonal antibody (Product # MA5-34613) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Product Details

MA5-34613

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-
Immunoprecipitation (IP)	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JU37-47
Immunogen	Recombinant protein within Human RbAP48 aa 1-98 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848521

Product Specific Information

Positive Control: NIH-3T3, Hela, rat brain tissue, mouse testis tissue, MCF-7, SH-SY5Y, human tonsil tissue, human lung cancer tissue, human liver tissue.

Target Information

RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; Histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; retinoblastoma binding protein 4; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48

Gene Aliases: lin-53; NURF55; RBAP48; RBBP4

UniProt ID: (Human) Q09028

Entrez Gene ID: (Human) 5928

Function(s)