Immunofluorescent analysis of Recoverin (green) showing staining in the cytoplasm of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Recoverin monoclonal antibody (Product # MA1-932) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Sheep, Rat|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-932 will detect Recoverin from human, rat, bovine, and ovine samples.
MA1-932 has been successfully used in Western blot, ICC/IF and IHC (P) procedures. By Western blot, this antibody will detect a ~23 kDa band representing Recoverin from rat brain, as well as in bovine and ovine retina. This antibody is not recommended for rat brain tissue in IHC applications.
The MA1-932 immunogen is considered commercially sensitive.
Calcium-binding proteins are implicated as being helper molecules in aberrant tissue growth of tumors. Recoverin is a neurologically important calcium binding protein, involved in tumor pathology. It is conserved across species and there are indication of several isoforms of this molecule. Recoverin antibodies are a useful tool for distinguishing between pineal gland-derived tissue ant the surrounding tissue of the brain. In pineal tumor tissue, the normal tissue staining pattern is lost, indicating the tissues loss of this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.