Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human pancreas stained using a Regenerating Islet-derived 1 polyclonal antibody (Product # PA5-23314) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (21 QGQESQTELPNPRISC 36) of Regenerating Islet-derived 1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Regenerating Islet-derived 1, also known as REG1, is a novel protein encoded by a multigene family comprising of REG and REG-related genes known to be involved in regeneration. REG1A and REG1B are the proteins encoded by these REG families of genes and belong to the pancreatic stone protein family. They are comprised of 166 amino acids and differed by only 22 amino acids with a C-type lectin domain. Known to be identical to that of pancreatic stone protein, REG proteins may be involved in the expansion of beta-cell mass during regeneration as well as in the maintenance of normal beta-cell function. These may act as inhibitors of spontaneous calcium carbonate precipitation and may also be associated with neuronal sprouting in brain, and with brain and pancreas (islet) regeneration. Expression of REG proteins is common in pancreas and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.