Western blot analysis of LGR7 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart lysate using a Relaxin Receptor 1/LGR7 polyclonal antibody (Product # PA1-41532) at 0.5 ug/ml (human), 1 ug/ml (mouse) and 2 ug/ml (rat).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 50-100 of human Relaxin receptor 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-41532 detects Relaxin receptor 1/LGR7 in monkey, chimpanzee, human, mouse and rat samples.
This antibody is expected to react with chicken, bovine, canine and opossum samples based on sequence homology.
LGR7 is a low density lipoprotein receptor of 757 amino acid belonging to the G-protein coupled receptor 1 family with rhodopsin-like receptor activity. It has seven transmembrane region, cysteine-rich motif, 10 LRR domains and 5 N-glycosylation sites in the N terminus. Research reports show that it has receptor for relaxins (RLN1 and RLN2) which stimulates adenylate cyclase activation, substrate adhesion, and chemo migratory capacity of mononuclear leukocytes. LGR7 is critical for mediating these biological responses by use of RNA interference lentiviral short hairpin constructs. It is widely expressed in 86% of 15 primary EC tissue samples including brain, kidney, testis and endometrium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.