PA1-1049 detects recombinant human resistin.
PA1-1049 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~7 kDa protein representing a 92 amino acid recombinant mouse resistin. This recombinant protein represents the immature monomeric form of resistin produced in E.coli. In its native form, resistin is a homodimer detectable at ~19 kDa.
The PA1-1049 immunogen is a synthetic peptide corresponding to residues C(22) S M E E A I N E R I Q E V A G S L(39) of human resistin. This peptide (Cat. # PEP-218) is available for use in neutralization and control experiments.
Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; cysteine-r; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; found in inflammatory zone 3; MGC126603; MGC126609; Resistin; resistin delta2; RSN
Gene Aliases: ADSF; FIZZ3; HXCP1; RETN; RETN1; RSTN; UNQ407/PRO1199; XCP1
UniProt ID: (Human) Q9HD89
Entrez Gene ID: (Human) 56729