|Tested species reactivity||Human, Pig, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of reticulon-1C.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50 - 1:100|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Western Blot (WB)||1:100 - 1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-06815 detects reticulon-1C(NSP-C) in human, porcine, and rat samples.
MA1-06815 has sucessfully been used in flow cytometry, Western blotting, immunocytochemistry and immunohistochemistry procedures. By Western blot it detects a ~23 kDa band representing reticulon-1C.
The MA1-06815 immunogen is a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of reticulon-1C.
Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight of ~135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of MANF as a protein interacting with RTN1-C.
MA1-06815 was used in immunocytochemistry, immunoprecipitation, and western blot to identify proteins that interact with RTN1-C
|Chen L,Wan L,Du J,Shen Y||Acta biochimica et biophysica Sinica (47:91)||2015|