Western blot analysis of Retinoblastoma was performed by loading 25 ug of K562 (Lane 1), B-3 (Lane 2) and rat eye (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Retinoblastoma monoclonal antibody (Product # MA5-11387) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 110 kDa in K562 and B-3 cells.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human Rb protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11384 targets Retinoblastoma in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and Mouse samples.
The MA5-11384 immunogen is recombinant human Rb protein.
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.
MA5-11384 was used in immunohistochemistry to study the lack of association of HPV with non-small cell lung cancer in Canadian and North American populations
|Yanagawa N,Wang A,Kohler D,Santos Gda C,Sykes J,Xu J,Pintilie M,Tsao MS||Lung cancer (Amsterdam, Netherlands) (79:215)||2013|
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
MA5-11384 was used in immunohistochemistry to study the role of the G1/S transition regulators in the recurrence of head and neck squamous carcinomas
|Canzonieri V,Barzan L,Franchin G,Vaccher E,Talamini R,Sulfaro S,Baldassarre G||Journal of cellular physiology (227:233)||2012|
Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue.
MA5-11384 was used in immunohistochemistry to study the prognositic value of alterations in the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue
|Panelos J,Beltrami G,Scoccianti G,Capanna R,Paglierani M,Pepi M,Massi D,Franchi A||Annals of surgical oncology (18:566)||2011|
Centrosome abnormalities in non-small cell lung cancer: correlations with DNA aneuploidy and expression of cell cycle regulatory proteins.
MA5-11384 was used in immunohistochemistry to investigate the correlations of DNA aneuploidy and expression of cell cycle regulatory proteins in non-small cell lung cancer
|Jung CK,Jung JH,Lee KY,Kang CS,Kim M,Ko YH,Oh CS||Pathology, research and practice (203:839)||2007|
The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance.
MA5-11384 was used in immunohistochemistry to study the clinical value of measuring retinoblastoma pathway expression in cervical intraepithelial neoplasia
|Nam EJ,Kim JW,Kim SW,Kim YT,Kim JH,Yoon BS,Cho NH,Kim S||Gynecologic oncology (104:207)||2007|
Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder.
MA5-11384 was used in immunohistochemistry to study the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder
|Yurakh AO,Ramos D,Calabuig-Fariñas S,López-Guerrero JA,Rubio J,Solsona E,Romanenko AM,Vozianov AF,Pellin A,Llombart-Bosch A||European urology (50:506)||2006|
Nucleolar size and activity are related to pRb and p53 status in human breast cancer.
MA5-11384 was used in immunohistochemistry to investigate the association of pRb and p53 status with nucleolar size and activity in human primary breast carcinomas
|Treré D,Ceccarelli C,Montanaro L,Tosti E,Derenzini M||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:1601)||2004|
The prognostic value of the AgNOR parameter in human breast cancer depends on the pRb and p53 status.
MA5-11384 was used in immunohistochemistry to investigate the association of pRb and p53 status with argyrophilic nucleolar organiser regions in human breast carcinoma
|Derenzini M,Ceccarelli C,Santini D,Taffurelli M,Treré D||Journal of clinical pathology (57:755)||2004|
p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.
MA5-11384 was used in immunohistochemistry to study the molecular determinants of the response to radiotherapy in oral carcinoma
|Jayasurya R,Francis G,Kannan S,Lekshminarayanan K,Nalinakumari KR,Abraham T,Abraham EK,Nair MK||International journal of cancer (109:710)||2004|
The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer.
MA5-11384 was used in immunohistochemistry to study p53, p16 and Rb protein expression and the clinicopathology of patients with locally advanced urinary bladder cancer
|Tzai TS,Tsai YS,Chow NH||Urologic oncology (22:112)||2004|
p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2.
MA5-11384 was used in immunohistochemistry to study the effects of epigenetic silencing of p14ARF on the subcellular localization of MDM2
|Esteller M,Cordon-Cardo C,Corn PG,Meltzer SJ,Pohar KS,Watkins DN,Capella G,Peinado MA,Matias-Guiu X,Prat J,Baylin SB,Herman JG||Cancer research (61:2816)||2001|
HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.
MA5-11384 was used in western blot to study the role of p16INK4A in the mechanism by which HFE polymorphisms affect the response of cancer cells to chemotherapy
|Lee SY,Liu S,Mitchell RM,Slagle-Webb B,Hong YS,Sheehan JM,Connor JR||International journal of cancer (129:2104)||2011|
The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways.
MA5-11384 was used in western blot to study the molecular mechanisms by which mesenchymal stem cells inhibit T cell proliferation
|Kim JA,Hong S,Lee B,Hong JW,Kwak JY,Cho S,Kim CC||Cellular immunology (245:16)||2007|
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
MA5-11384 was used in western blot to study the antiproliferative and apoptotic effects of ixocarpalactone A on colon cancer cells
|Choi JK,Murillo G,Su BN,Pezzuto JM,Kinghorn AD,Mehta RG||The FEBS journal (273:5714)||2006|
Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo.
MA5-11384 was used in western blot to study the inhibition of C6 glioma cell growth by caffeic acid phenethyl ester and the potential mechanisms involved
|Kuo HC,Kuo WH,Lee YJ,Lin WL,Chou FP,Tseng TH||Cancer letters (234:199)||2006|
Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit.
MA5-11384 was used in western blot to study the role of ribonucleotide reductase M2 subunit downregulation in the mechanism by which flavopiridol sensitizes gastrointestinal cancer cell lines to gemcitabine
|Jung CP,Motwani MV,Schwartz GK||Clinical cancer research : an official journal of the American Association for Cancer Research (7:2527)||2001|
Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines.
MA5-11384 was used in immunocytochemistry and western blot to study the effects of rRNA synthesis inhibition on cell cycle progression and cell population growth according to retinoblastoma and p53 status
|Montanaro L,Mazzini G,Barbieri S,Vici M,Nardi-Pantoli A,Govoni M,Donati G,Treré D,Derenzini M||Cell proliferation (40:532)||2007|