Western blot analysis of Retinoic Acid Receptor gamma-2 was performed by loading 25 ug of SK-BR-3 (lane 1) and Hela (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Retinoic Acid Receptor gamma-2 polyclonal antibody (Product # PA3-814) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~50kDa.
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(13) R L Y G A A G P G A G L L R R(28) of the N-terminal sequence of RAR gamma-2.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1-2 µl|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-814 detects recombinant human retinoic acid receptor (RAR) gamma 2. The immunizing sequence is 100% conserved in mouse. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR.
PA3-814 has been successfully used in WB, immunoprecipitation and gel shift procedures. In super shift assays this antibody incompletely shifts human RAR gamma-2/retinoid X receptor (RXR)/RAR response element complexes using various synthetic and natural RARE's.
PA3-814 immunizing peptide corresponds to the N-terminus of RAR gamma-2. This antibody does not detect RAR gamma-1, or either of the alpha or beta forms of RAR. This peptide (Cat. # PEP-229) is available for use in neutralization and control experiments.
Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD.
The corresponding gene for the retinoic acid receptor gamma 2 is NR1B3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Retinoic acid receptor gamma 2 interactions with vitamin D response elements.
PA3-814 was used in western blot to study the interaction between retinoic acid receptor gamma 2 and vitamin D response elements
|Koszewski NJ,Herberth J,Malluche HH||The Journal of steroid biochemistry and molecular biology (120:200)||2010|
The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.
PA3-814 was used in immunohistochemistry to study the expression of retinoic acid receptor alpha in the granule cells of schizophrenic dentate gyrus
|Rioux L,Arnold SE||Psychiatry research (133:13)||2005|