|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rabbit, Non-human primate, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human retinoic X receptor beta protein.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-812 detects retinoid X receptor (RXR) beta from human and mouse tissues. This antibody is specific for the RXR beta isoform and does not cross react with either RXR alpha or gamma.
MA3-812 has been successfully used in Western blot, immunofluorescence, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from transfected Sf9 cells. Immunofluorescence staining of RXR beta in MCF-7 cells overexpressing RXR beta with MA3-812 results in strong nuclear staining. Gel super shift experiments show disruption of vitamin D receptor (VDR)/RXR beta/VDRE (DNA) complex formation when RXR beta was preincubated with MA3-812, yet experiments using THR showed a supershift of the thyroid hormone receptor (THR)/RXR beta heterodimer by this product.
The MA3-812 antigen is recombinant human RXR beta.
Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta, also known as RCoR1, controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).
The corresponding gene for the retinoid X receptor beta is NR2B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
G2/M arrest by 1,25-dihydroxyvitamin D3 in ovarian cancer cells mediated through the induction of GADD45 via an exonic enhancer.
MA3-812 was used in EMSA to study the role of GADD45 in tumor-suppressing activity of 1,25-dihydroxyvitamin D3 in human ovarian cancer.
|Jiang F,Li P,Fornace AJ,Nicosia SV,Bai W||The Journal of biological chemistry (278:48030)||2003|
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
MA3-812 was used in EMSA assay to investigate the effect of 1,25-(OH)2D3 and 9-cis-RA on the binding of VDR and RXR with VDREs
|Thompson PD,Jurutka PW,Haussler CA,Whitfield GK,Haussler MR||The Journal of biological chemistry (273:8483)||1998|
Heterodimerization of thyroid hormone (TH) receptor with H-2RIIBP (RXR beta) enhances DNA binding and TH-dependent transcriptional activation.
MA3-812 was used in EMSA assay to investigate the interaction between THR alpha and RXR beta and their corresponding functions
|Hallenbeck PL,Marks MS,Lippoldt RE,Ozato K,Nikodem VM||Proceedings of the National Academy of Sciences of the United States of America (89:5572)||1992|
A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR.
MA3-812 was used in EMSA assay to study the role of RXA and RAR in the regulation of cellular retinol-binding protein type II gene
|Mangelsdorf DJ,Umesono K,Kliewer SA,Borgmeyer U,Ong ES,Evans RM||Cell (66:555)||1991|
|Non-human primate||Not Cited||
Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions.
MA3-812 was used in western blot to study the agonist-stimulated RXR degradation
|Osburn DL,Shao G,Seidel HM,Schulman IG||Molecular and cellular biology (21:4909)||2001|