|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide: D(47) H M I I R T L S T F R N Y I M D F Q(65)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-727 detects Retinol Binding Protein 1 in rat samples.
PA1-727 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~21 kDa protein representing Retinol Binding Protein 1 in rat liver, lung, brain and testis samples.
PA1-727 immunizing peptide corresponds to amino acid residues 47-65 of rat RBP 1. This sequence is conserved across many species including mouse, human and bovine. This peptide (Cat. # PEP-285) is available for use in neutralization and control experiments.
The cellular retinol binding proteins (CRBPs) are an important component of retinoid activity, and involved in the transport and metabolism of retinoids. In humans, three known isoforms exist: CRBP1, CRBP2 and CRBP3.
Although the function of the retinol binding proteins are not fully understood, it is thought they assist in the formation of retinyl esters for storage or conversion of retinol to retinoic acid. Cellular retinol-binding protein-1 (CRBP-1, RBP1 or Retinol Binding Protein 1) is involved in the transport of Vitamin A from the liver to the peripheral tissue. CRBP-1 is highly expressed in liver cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.