|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||Assay dependent|
|Tested Species reactivity||Fungi|
|Host / Isotype||Mouse / IgM|
|Immunogen||Water-soluble somatic antigens from Rhizopus arrhizus.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This clone recognizes Rhizopus arrhizus and other members of the family Mucoraceae including Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present. It has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components. This clone does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
In Western blot, this antibody detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens. IHC staining requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. Recommended postive control: lymph nodes from R. arrhizus infected cattle.
R. arrhizus is an angio-invasive filamentous fungus. It is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: R. Arrhizus
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