|Tested species reactivity||Human, Mouse, Non-human primate, Rabbit, Rat|
|Host / Isotype||Mouse / IgG|
|Immunogen||Fusion protein corresponding to residues 180-610 of rat RhoGAP.|
|Storage buffer||0.07M tris glycine, pH 7.4, with 0.105M NaCl|
|Contains||0.035% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||4 µg|
|Western Blot (WB)||0.5 - 1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25557 detects RhoGAP from human, mouse, rat, monkey, and rabbit samples.
MA1-25557 has been successfully used in Immunoprecipitation, and Western blot procedures.
The MA1-25557 immunogen is a fusion protein corresponding to residues 180-610 of rat RhoGAP.
The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.