Invitrogen

Rhodopsin Monoclonal Antibody (1D4)

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Antibody Testing Data (5)
Published Figures (7)

Rhodopsin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

Rhodopsin Antibody (MA1-722) in IHC (P)

Immunohistochemistry analysis of Rhodopsin showing staining in the nucleus of paraffin-embedded mouse retinal tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature,... View More

Rhodopsin Antibody in Immunohistochemistry (IHC)

Rhodopsin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

MA1-722

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

View 6 publications 6 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Dot blot (DB)

View 1 publication 1 publication

Affinity Purification (AP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D4

Immunogen

Bovine rhodopsin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325050

Product Specific Information

MA1-722 detects rhodopsin from human, mouse and bovine retinal samples. Clone 1D4 has also been used successfully in Zebrafish. It does not recognize rhodopsin in zebrafish rods, but instead labels long double cone outer segments that express red opsin (Yin, J. et al, 2012).

MA1-722 has been successfully used in Western blot, IHC (P), immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~40 kDa protein representing rhodopsin from Sf9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.

The MA1-722 immunogen is bovine rhodopsin. The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.

Target Information

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intramembrane client recognition potentiates the chaperone functions of calnexin.

The EMBO journal

Bloemeke N,Meighen-Berger K,Hitzenberger M,Bach NC,Parr M,Coelho JP,Frishman D,Zacharias M,Sieber SA,Feige MJ

Published figure using Rhodopsin monoclonal antibody (Product # MA1-722) in Western Blot

Thu Dec 15 00:00:00 EST 2022

Effects of Epigenetic Modification of PGC-1α by a Chemical Chaperon on Mitochondria Biogenesis and Visual Function in Retinitis Pigmentosa.

Cells

Ozawa Y,Toda E,Homma K,Osada H,Nagai N,Tsubota K,Okano H

Published figure using Rhodopsin monoclonal antibody (Product # MA1-722) in Immunohistochemistry (Paraffin)

Fri Apr 29 00:00:00 EDT 2022

Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants.

Nature communications

Tian S,Xiong X,Zeng J,Wang S,Tremblay BJ,Chen P,Chen B,Liu M,Chen P,Sheng K,Zeve D,Qi W,Breault DT,Rodríguez C,Gerhard R,Jin R,Doxey AC,Dong M

MA1-722 was used in Immunocytochemistry-immunofluorescence to identify TFPI as a TcdB receptor and reveal IR-driven changes on receptor-specificity among TcdB variants.

Wed Nov 09 00:00:00 EST 2022

Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2.

Viruses

Harte JV,Wakerlin SL,Lindsay AJ,McCarthy JV,Coleman-Vaughan C

MA1-722 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest a benefit to the incorporation of pharmacological inhibitors of metalloproteases into treatment strategies for patients with COVID-19.

Wed Sep 21 00:00:00 EDT 2022

Functional analyses of epidemic Clostridioides difficile toxin B variants reveal their divergence in utilizing receptors and inducing pathology.

PLoS pathogens

Pan Z,Zhang Y,Luo J,Li D,Zhou Y,He L,Yang Q,Dong M,Tao L

MA1-722 was used in Western Blot to demonstrate divergence in the receptor preference and further lead to colonic pathology for predominant Clostridioides difficile toxin B subtypes.

Fri Jan 01 00:00:00 EST 2021

Deletion of Asrgl1 Leads to Photoreceptor Degeneration in Mice.

Frontiers in cell and developmental biology

Zhou Y,Tian W,Jiang X,Yang H,Jiang Z,Li X,Jiang D,Sun K,Yang Y,Liu W,Zhu X

MA1-722 was used in Immunohistochemistry to provide a knockout mouse model for a better understanding of the molecular mechanism for ASRGL1-related RP.

Sat Feb 05 00:00:00 EST 2022

Neuroprotective Effect of 4-Phenylbutyric Acid against Photo-Stress in the Retina.

Antioxidants (Basel, Switzerland)

Guzmán Mendoza NA,Homma K,Osada H,Toda E,Ban N,Nagai N,Negishi K,Tsubota K,Ozawa Y

MA1-722 was used in Immunohistochemistry to analyse functional, histological, and molecular changes in the light-exposed retina and the effects of administering 4-phenylbutyric acid in mice.

Tue Jul 20 00:00:00 EDT 2021

Neuroprotective Effect of 4-Phenylbutyric Acid against Photo-Stress in the Retina.

Antioxidants (Basel, Switzerland)

Guzmán Mendoza NA,Homma K,Osada H,Toda E,Ban N,Nagai N,Negishi K,Tsubota K,Ozawa Y

MA1-722 was used in Immunohistochemistry to analyse functional, histological, and molecular changes in the light-exposed retina and the effects of administering 4-phenylbutyric acid in mice.

Tue Jul 20 00:00:00 EDT 2021

Genome-Wide CRISPR Screen Identifies Semaphorin 6A and 6B as Receptors for Paeniclostridium sordellii Toxin TcsL.

Cell host & microbe

Tian S,Liu Y,Wu H,Liu H,Zeng J,Choi MY,Chen H,Gerhard R,Dong M

MA1-722 was used in Immunocytochemistry to establish SEMA6A and 6B as pathophysiologically relevant receptors for TcsL.

Wed May 13 00:00:00 EDT 2020

SARS-CoV-2 and Three Related Coronaviruses Utilize Multiple ACE2 Orthologs and Are Potently Blocked by an Improved ACE2-Ig.

Journal of virology

Li Y,Wang H,Tang X,Fang S,Ma D,Du C,Wang Y,Pan H,Yao W,Zhang R,Zou X,Zheng J,Xu L,Farzan M,Zhong G

MA1-722 was used in Western Blotting to provide a promising approach to developing antiviral proteins broadly effective against these distinct coronaviruses.

Tue Oct 27 00:00:00 EDT 2020

Deletion of the Impg2 gene causes the degeneration of rod and cone cells in mice.

Human molecular genetics

Xu H,Qu C,Gan L,Sun K,Tan J,Liu X,Jiang Z,Tian W,Liu W,Zhang S,Yang Y,Jiang L,Zhu X,Zhang L

MA1-722 was used in Immunohistochemistry to develop two independent Impg2 knockout (KO) mouse models using the CRISPR/Cas9 technique to assess the in vivo functions of Impg2 in the retina.

Sat Jun 27 00:00:00 EDT 2020

Metipranolol promotes structure and function of retinal photoreceptors in the rd10 mouse model of human retinitis pigmentosa.

Journal of neurochemistry

Kanan Y,Khan M,Lorenc VE,Long D,Chadha R,Sciamanna J,Green K,Campochiaro PA

Published figure using Rhodopsin monoclonal antibody (Product # MA1-722) in Immunofluorescence

Tue Jan 01 00:00:00 EST 2019

Role of Translational Attenuation in Inherited Retinal Degeneration.

Investigative ophthalmology & visual science

Starr CR,Nyankerh CNA,Qi X,Hu Y,Gorbatyuk OS,Sonenberg N,Boulton ME,Gorbatyuk MS

Published figure using Rhodopsin monoclonal antibody (Product # MA1-722) in Immunofluorescence

Fri Nov 01 00:00:00 EDT 2019

Role of Translational Attenuation in Inherited Retinal Degeneration.

Investigative ophthalmology & visual science

Starr CR,Nyankerh CNA,Qi X,Hu Y,Gorbatyuk OS,Sonenberg N,Boulton ME,Gorbatyuk MS

Published figure using Rhodopsin monoclonal antibody (Product # MA1-722) in Immunofluorescence

Fri Nov 01 00:00:00 EDT 2019

Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.

Nature

Kang Y,Kuybeda O,de Waal PW,Mukherjee S,Van Eps N,Dutka P,Zhou XE,Bartesaghi A,Erramilli S,Morizumi T,Gu X,Yin Y,Liu P,Jiang Y,Meng X,Zhao G,Melcher K,Ernst OP,Kossiakoff AA,Subramaniam S,Xu HE

MA1-722 was used in Immunocytochemistry-immunoflourescence to show that the major interactions between activated rhodopsin and Gi are mediated by the C-terminal helix of the Gi α-subunit, which is wedged into the cytoplasmic cavity of the transmembrane helix bundle and directly contacts the amino terminus of helix 8 of rhodopsin.

Fri Jun 01 00:00:00 EDT 2018

Whole-exome sequencing revealed HKDC1 as a candidate gene associated with autosomal-recessive retinitis pigmentosa.

Human molecular genetics

Zhang L,Sun Z,Zhao P,Huang L,Xu M,Yang Y,Chen X,Lu F,Zhang X,Wang H,Zhang S,Liu W,Jiang Z,Ma S,Chen R,Zhao C,Yang Z,Sui R,Zhu X

MA1-722 was used in Immunohistochemistry-immunofluorescence to demonstrate how HKDC1 is associated with autosomal recessively inherited retinitis pigmentosa.

Sat Dec 01 00:00:00 EST 2018

A G Protein-Coupled Receptor Dimerization Interface in Human Cone Opsins.

Biochemistry

Jastrzebska B,Comar WD,Kaliszewski MJ,Skinner KC,Torcasio MH,Esway AS,Jin H,Palczewski K,Smith AW

MA1722 was used in western blot to study the dimerization affinity and binding interface of human cone opsins

Tue Jan 10 00:00:00 EST 2017

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B.

Nature

Tao L,Zhang J,Meraner P,Tovaglieri A,Wu X,Gerhard R,Zhang X,Stallcup WB,Miao J,He X,Hurdle JG,Breault DT,Brass AL,Dong M

MA1722 was used in immunocytochemistry to report that frizzled family proteins are receptors for Clostridium difficile toxin B

Thu Oct 20 00:00:00 EDT 2016

Peripherin-2 couples rhodopsin to the CNG channel in outer segments of rod photoreceptors.

Human molecular genetics

Becirovic E,Nguyen ON,Paparizos C,Butz ES,Stern-Schneider G,Wolfrum U,Hauck SM,Ueffing M,Wahl-Schott C,Michalakis S,Biel M

MA1-722 was used in Immunoprecipitation to identify additional proteins of the rod cyclic nucleotide-gated channel that interact with the CNGB1a core subunit.

Sat Nov 15 00:00:00 EST 2014

Mosaic synaptopathy and functional defects in Cav1.4 heterozygous mice and human carriers of CSNB2.

Human molecular genetics

Michalakis S,Shaltiel L,Sothilingam V,Koch S,Schludi V,Krause S,Zeitz C,Audo I,Lancelot ME,Hamel C,Meunier I,Preising MN,Friedburg C,Lorenz B,Zabouri N,Haverkamp S,Garcia Garrido M,Tanimoto N,Seeliger MW,Biel M,Wahl-Schott CA

MA1-722 was used in Immunohistochemistry to compare retinal phenotypes in Cav1.4-deficient and Cav1.4 heterozygous mice and in human female carrier patients.

Sat Mar 15 00:00:00 EDT 2014

1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins.

Methods in molecular biology (Clifton, N.J.)

Molday LL,Molday RS

MA1-722 was used in immunocytochemistry and western blot to describe various immunochemical procedures used for the detection, purification, and localization of 1D4-tagged proteins

Thu Feb 12 00:00:00 EST 2015

Human melanopsin forms a pigment maximally sensitive to blue light (λmax ≈ 479 nm) supporting activation of G(q/11) and G(i/o) signalling cascades.

Proceedings. Biological sciences

Bailes HJ,Lucas RJ

MA1-722 was used in Western Blotting to investigate the physiological responses driven by human melanopsin under heterologous expression in HEK293 cells.

Wed May 22 00:00:00 EDT 2013

Reproducible and sustained regulation of Gαs signalling using a metazoan opsin as an optogenetic tool.

PloS one

Bailes HJ,Zhuang LY,Lucas RJ

MA1-722 was used in immunocytochemistry to evaluate a novel optogenetic tool for the study of G protein-coupled receptor signalling

Mon Jun 04 00:00:00 EDT 2012

Assays for inverse agonists in the visual system.

Methods in enzymology

Kono M

MA1-722 was used in Dot blot to describe a method for determining the effects of ligands on rod and cone opsin activity.

Fri Mar 11 00:00:00 EST 2011

In vitro assays of rod and cone opsin activity: retinoid analogs as agonists and inverse agonists.

Methods in molecular biology (Clifton, N.J.)

Kono M,Crouch RK

MA1-722 was used in Immunopurification to demonstrate an in vitro assay for determining the ability of a retinal analog to act as an agonist or an inverse agonist with various opsins.

Thu Sep 09 00:00:00 EDT 2010

TSPAN12 regulates retinal vascular development by promoting Norrin- but not Wnt-induced FZD4/beta-catenin signaling.

Cell

Junge HJ,Yang S,Burton JB,Paes K,Shu X,French DM,Costa M,Rice DS,Ye W

MA1-722 was used in western blot to study the mechanism by which TSPAN12 regulates retinal vascular development

Fri Oct 16 00:00:00 EDT 2009

High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.

Journal of lipid research

Tanito M,Brush RS,Elliott MH,Wicker LD,Henry KR,Anderson RE

MA1-722 was used in western blot to study the effect of retinal membrane docosahexaenoic acid on stress-induced retinal degeneration in mice.

Fri May 01 00:00:00 EDT 2009

High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.

Journal of lipid research

Tanito M,Brush RS,Elliott MH,Wicker LD,Henry KR,Anderson RE

MA1-722 was used in western blot to study the effect of retinal membrane docosahexaenoic acid on stress-induced retinal degeneration in mice.

Fri May 01 00:00:00 EDT 2009

Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats.

Investigative ophthalmology & visual science

Tanito M,Kaidzu S,Anderson RE

MA1-722 was used in western blot to investigate the effects of acute light exposure on rat retina and choroid

Sun Apr 01 00:00:00 EDT 2007

A neural-specific F-box protein Fbs1 functions as a chaperone suppressing glycoprotein aggregation.

The Journal of biological chemistry

Yoshida Y,Murakami A,Iwai K,Tanaka K

MA1-722 was used in immunoprecipitation to investigate the function of a neural-specific F-box protein Fbs1 in glycoprotein aggregation.

Fri Mar 09 00:00:00 EST 2007

RPGRIP1s with distinct neuronal localization and biochemical properties associate selectively with RanBP2 in amacrine neurons.

Human molecular genetics

Castagnet P,Mavlyutov T,Cai Y,Zhong F,Ferreira P

MA1-722 was used in western blot to investigate the distribution of RPGRIP1s and their association with RanBP2 in amacrine neurons

Fri Aug 01 00:00:00 EDT 2003

Alleviation of constant-light-induced photoreceptor degeneration by adaptation of adult albino rat to bright cyclic light.

Investigative ophthalmology & visual science

Li F,Cao W,Anderson RE

MA1-722 was used in western blot to demonstrate the rapid response of adult rat retina to light change.

Sat Nov 01 00:00:00 EST 2003

Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle.

Nature genetics

Redmond TM,Yu S,Lee E,Bok D,Hamasaki D,Chen N,Goletz P,Ma JX,Crouch RK,Pfeifer K

MA1-722 was used in western blot to study the role of RPE65 in 11-cis-vitamin A production in retinal visual cycle

Tue Dec 01 00:00:00 EST 1998

Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man.

Proceedings of the National Academy of Sciences of the United States of America

Cideciyan AV,Hood DC,Huang Y,Banin E,Li ZY,Stone EM,Milam AH,Jacobson SG

MA1-722 was used in immunocytochemistry to study the mechanism for rod and cone photoreceptor degeneration from rhodopsin mutations

Tue Jun 09 00:00:00 EDT 1998

Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.

The Journal of biological chemistry

Hodges RS,Heaton RJ,Parker JM,Molday L,Molday RS

MA1-722 was used in ELISA to investigate the specificity of monoclonal anti-rhodopsin antibodies

Thu Aug 25 00:00:00 EDT 1988

Bioinformatics

Protein Aliases: L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; Red Opsin; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); Rod Opsin; RP 4

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Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4

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UniProt ID: (Bovine) P02699, (Human) P08100, (Rat) P51489, (Mouse) P15409

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Entrez Gene ID: (Bovine) 509933, (Human) 6010, (Rat) 24717, (Mouse) 212541

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Function(s)

G-protein alpha-subunit binding opsin binding guanyl-nucleotide exchange factor activity 11-cis retinal binding protein binding G-protein coupled photoreceptor activity zinc ion binding identical protein binding arrestin family protein binding G-protein coupled receptor activity retinal binding metal ion binding photoreceptor activity spectrin binding signal transducer activity G-protein coupled receptor

Process(es)

protein phosphorylation visual perception phototransduction, visible light response to light stimulus absorption of visible light rhodopsin mediated signaling pathway protein-chromophore linkage photoreceptor cell maintenance retina development in camera-type eye retinoid metabolic process G-protein coupled receptor signaling pathway red, far-red light phototransduction regulation of rhodopsin mediated signaling pathway phototransduction detection of light stimulus sensory perception of light stimulus cellular response to light stimulus signal transduction response to stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Rhodopsin Monoclonal Antibody (1D4)

Rhodopsin Antibody (MA1-722) in IHC (P)

Product Details

MA1-722

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Affinity Purification (AP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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