Invitrogen

Rhotekin Monoclonal Antibody (OTI2E9)

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Rhotekin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a RTKN monoclonal antibody (Product # MA5-26050).

Rhotekin Antibody in Western Blot (WB) — Western blot analysis of RTKN in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with RTKN (Product # MA5-26050) monoclonal antibody.

Rhotekin Antibody in Western Blot, Immunoprecipitation (WB, IP) — 5 Figure Rhotekin couples RhoA to S100A4 and NM myosin II in SM cells and tissues stimulated with ACh A , rhotekin (RTKN) was immunoprecipitated from tissue extracts and immunocomplexes were blotted for RhoA, S100A4, RTKN and NM myosin IIA. Stimulation for 5 min with 10 -5 M ACh significantly increased the coprecipitation of NM myosin IIA ( n = 6, p = 0.0070), RhoA ( n = 3, p = 0.0359) and S100A4 ( n = 9, p = 0.0001) with RTKN. B , in situ PLA was used to determine the interaction of RTKN and RhoA, S100A4 and NM myosin IIA in freshly dissociated tracheal SM cells. Stimulation with ACh caused a significant increase in the number of PLA complexes between all four proteins (RTKN-RhoA, n = 20, p = 0.0001; RTKN-S100A4, n = 20, p = 0.0001; RTKN-NM myosin IIA, n = 31, p = 0.0001). C , tracheal SM tissues were treated with RTKN siRNA. Depletion of RTKN significantly inhibited protein expression ( n = 10, p = 0.0001) and also inhibited tension development in response to 10 -5 M ACh stimulation ( n = 16, p = 0.0001). D , PLA shows interactions between S100A4 and RhoA in cells dissociated from sham-treated tissues and from RTKN-depleted tissues. ACh stimulation of sham-treated cells resulted in a significant increase in the interactions between S100A4 and RhoA (US, n = 16; ACh, n = 20). RTKN depletion inhibited the ACh-induced increase of the interaction between S100A4 and RhoA ( p = 0.0001) (US, n = 18; ACh, n = 19). Data analysed by a paired Student's t test ( A and C ), an unpaired S

Product Details

MA5-26050

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Dog
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI2E9
Immunogen	Full length human recombinant protein of RTKN produced in HEK293T cell View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2725236

Target Information

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.

The Journal of physiology

Zhang W,Gunst SJ

MA5-26050 was used in Immunoprecipitation, Western Blot to test the hypothesis that S100A4 contributes to the regulation of contraction in airway smooth muscle by regulating a pool of non-muscle myosin II at the cell cortex.

Thu Oct 01 00:00:00 EDT 2020

S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.

The Journal of physiology

Zhang W,Gunst SJ

Thu Oct 01 00:00:00 EDT 2020

S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.

The Journal of physiology

Zhang W,Gunst SJ

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Rhotekin; RTKN

Gene Aliases: RTKN; RTKN1

UniProt ID: (Human) Q9BST9

Entrez Gene ID: (Human) 6242

Molecular Function: non-motor actin binding protein

Function(s)

apoptotic process signal transduction Rho protein signal transduction negative regulation of GTPase activity

Process(es)

GTPase inhibitor activity protein binding GTP binding GTP-Rho binding

Rhotekin Monoclonal Antibody (OTI2E9)

Product Details

MA5-26050

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!