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Immunofluorescent analysis of Ribonuclease A showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Ribonuclease A polyclonal antibody (Product # PA5-28278) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 156 of Human Ribonuclease A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28278 targets Ribonuclease A in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28278 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 156 of Human Ribonuclease A.
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HP-RNase; RIB-1; RIB1; ribonuclease 1; ribonuclease A; ribonuclease A C1; ribonuclease pancreatic; ribonuclease, RNase A family, 1 (pancreatic); RNase 1; RNase A; RNase upI-1; RNS1
RAC1; RIB1; RNASE1; RNS1