Western blot analysis showing siRNA knock-down of Ribosome binding protein 1 using 30ug HepG2 (A), RRBP1 siRNA#1-transfected HepG2 (B) and RRBP1 siRNA#2-transfected HepG2 (C) whole cell lysates. Detection was performed using a Ribosome binding protein 1 monoclonal antibody (Product # MA5-18289) at a dilution of 1:1000 (upper panel) and a Vinculin polyclonal antibody at a dilution of 1:1000 (lower panel) on 5% SDS-PAGE.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 750 and 977 of Ribosome binding protein 1|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.