Western blot analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using a RuvB-like 1 polyclonal antibody (Product # PA5-23451) at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||portion of amino acids 1-50 of human Rvb1|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Rvbs have been implicated in human pathologies, including carcinogenesis. For example Rvb1 overexpresssion has been reported in colon carcinoma, and Rvb1 SUMOylation has been shown to increase the proliferation and growth of prostate cancer cells. Rvb1 may also play a role in autoimmunity as high levels of RUVB1 autoantibodies have been detected in the sera of patients with autoimmune diseases including polymyositis/dermatomyosistic and autoimmune hepatitis. The extensive involvement of Rvbs in DNA-associated processes, including their interaction with a wide range of transcription factors, suggests that Rvb dysregulation may be involved in a number of other pathological processes yet to be elucidated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.