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Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Ryanodine Receptor (MA3-925) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Amphibian, Bovine, Dog, Chicken, Fish, Human, Mustelid, Mouse, Non-human primate, Sheep, Rabbit, Rat|
|Published species reactivity||Dog, Rabbit, Rat, Pig, Bovine, Human, Mouse, Chicken, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified chicken pectoral muscle ryanodine receptor.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:1,000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, MA3-925 detects the alpha and beta isoforms and in fish it detects the alpha isoform.
MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.
The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.
The ryanodine receptor (RyR) is the channel responsible for calcium release from muscle cell sarcoplasmic reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR.
Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.
MA3-925 was used in western blot to study SERCA dysfunction in mice overexpressing phospholamban
|Fajardo VA,Bombardier E,McMillan E,Tran K,Wadsworth BJ,Gamu D,Hopf A,Vigna C,Smith IC,Bellissimo C,Michel RN,Tarnopolsky MA,Quadrilatero J,Tupling AR||Disease models & mechanisms (8:999)||2015|
Compound RYR1 heterozygosity resulting in a complex phenotype of malignant hyperthermia susceptibility and a core myopathy.
MA3-925 was used in western blot to determine which RYR1 variants contribute to malignant hyperthermia
|Kraeva N,Heytens L,Jungbluth H,Treves S,Voermans N,Kamsteeg E,Ceuterick-de Groote C,Baets J,Riazi S||Neuromuscular disorders : NMD (25:567)||2015|
Mitsugumin 56 (hedgehog acyltransferase-like) is a sarcoplasmic reticulum-resident protein essential for postnatal muscle maturation.
MA3-925 was used in western blot to investigate Mitsugumin 56 in skeletal muscle maturation using knockout mice
|Van B,Nishi M,Komazaki S,Ichimura A,Kakizawa S,Nakanaga K,Aoki J,Park KH,Ma J,Ueyama T,Ogata T,Maruyama N,Takeshima H||FEBS letters (589:1095)||2015|
Suppression of InsP3 receptor-mediated Ca2+ signaling alleviates mutant presenilin-linked familial Alzheimer's disease pathogenesis.
MA3-925 was used in western blot to study the role of exagerated Ca(2+) signaling mediated by InsP3 receptor1 in the pathogenesis of mutant presenilin-linked Alzheimer's disease
|Shilling D,Müller M,Takano H,Mak DO,Abel T,Coulter DA,Foskett JK||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:6910)||2014|
The ryanodine receptor store-sensing gate controls Ca2+ waves and Ca2+-triggered arrhythmias.
MA3-925 was used in western blot to study the role of the ryanodine receptor store-sensing gate in ventricular tachyarrhythmias
|Chen W,Wang R,Chen B,Zhong X,Kong H,Bai Y,Zhou Q,Xie C,Zhang J,Guo A,Tian X,Jones PP,O'Mara ML,Liu Y,Mi T,Zhang L,Bolstad J,Semeniuk L,Cheng H,Zhang J,Chen J,Tieleman DP,Gillis AM,Duff HJ,Fill M,Song LS,Chen SR||Nature medicine (20:184)||2014|
In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes.
MA3-925 was used in western blot to study Ca(2+) handling in diabetic rat skeletal muscle using in vivo imaging
|Eshima H,Tanaka Y,Sonobe T,Inagaki T,Nakajima T,Poole DC,Kano Y||American journal of physiology. Regulatory, integrative and comparative physiology (305:R610)||2013|
Variable myopathic presentation in a single family with novel skeletal RYR1 mutation.
MA3-925 was used in western blot to investigate a novel skeletal RYR1 mutation in an autosomal recessive heterogeneous congenital myopathy
|Attali R,Aharoni S,Treves S,Rokach O,Becker Cohen M,Fellig Y,Straussberg R,Dor T,Daana M,Mitrani-Rosenbaum S,Nevo Y||PloS one (8:null)||2013|
Novel excitation-contraction uncoupled RYR1 mutations in patients with central core disease.
MA3-925 was used in western blot to functionally characterize 5 novel mutations of RYR1 from patients with central core disease
|Kraeva N,Zvaritch E,Rossi AE,Goonasekera SA,Zaid H,Frodis W,Kraev A,Dirksen RT,Maclennan DH,Riazi S||Neuromuscular disorders : NMD (23:120)||2013|
Identification of ATP-binding regions in the RyR1 Ca²⁺ release channel.
MA3-925 was used in western blot to use a photo-activated ATP analogue to identify the regions of the RyR1 receptor that bind ATP
|Popova OB,Baker MR,Tran TP,Le T,Serysheva II||PloS one (7:null)||2012|
Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?
MA3-925 was used in western blot to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure
|Middlekauff HR,Vigna C,Verity MA,Fonarow GC,Horwich TB,Hamilton MA,Shieh P,Tupling AR||Journal of cardiac failure (18:724)||2012|
Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle.
MA3-925 was used in western blot to study the effect of dietary nitrate on Ca2+ handling and contractile force in mouse fast-twitch muscle
|Hernández A,Schiffer TA,Ivarsson N,Cheng AJ,Bruton JD,Lundberg JO,Weitzberg E,Westerblad H||The Journal of physiology (590:3575)||2012|
Tirapazamine-doxorubicin interaction referring to heart oxidative stress and Ca²⁺ balance protein levels.
MA3-925 was used in western blot to study the effect of tirapazamine on oxidative stress and heart contractile protein level in doxorubicin-treated rats
|Sliwinska J,Dudka J,Korga A,Burdan F,Matysiak W,Jodlowska-Jedrych B,Mandziuk S,Dawidek-Pietryka K||Oxidative medicine and cellular longevity (2012:null)||2012|
SRP-35, a newly identified protein of the skeletal muscle sarcoplasmic reticulum, is a retinol dehydrogenase.
MA3-925 was used in western blot to study the role of a newly identified retinol dehydrogenase in skeletal muscle sarcoplasmic reticulum
|Treves S,Thurnheer R,Mosca B,Vukcevic M,Bergamelli L,Voltan R,Oberhauser V,Ronjat M,Csernoch L,Szentesi P,Zorzato F||The Biochemical journal (441:731)||2012|
Decreased cardiac L-type Ca²⁺ channel activity induces hypertrophy and heart failure in mice.
MA3-925 was used in western blot to study the effect of cardiac L-type calcium channel inhibitors on cardiomyocytes and vasculature
|Goonasekera SA,Hammer K,Auger-Messier M,Bodi I,Chen X,Zhang H,Reiken S,Elrod JW,Correll RN,York AJ,Sargent MA,Hofmann F,Moosmang S,Marks AR,Houser SR,Bers DM,Molkentin JD||The Journal of clinical investigation (122:280)||2012|
Role of presenilin 1 in structural plasticity of cortical dendritic spines in vivo.
MA3-925 was used in western blot to study the role of presenilin-1 in cortical dendritic spine plasticity in vivo
|Jung CK,Fuhrmann M,Honarnejad K,Van Leuven F,Herms J||Journal of neurochemistry (119:1064)||2011|
Integrity of the network sarcoplasmic reticulum in skeletal muscle requires small ankyrin 1.
MA3-925 was used in western blot to investigate the role of small ankyrin 1 in sarcoplasmic reticulum of skeletal muscle
|Ackermann MA,Ziman AP,Strong J,Zhang Y,Hartford AK,Ward CW,Randall WR,Kontrogianni-Konstantopoulos A,Bloch RJ||Journal of cell science (124:3619)||2011|
Ryanodine receptor oxidation causes intracellular calcium leak and muscle weakness in aging.
MA3-925 was used in western blot to determine the role of Ryanodine receptors in age-related loss of muscle functioning
|Andersson DC,Betzenhauser MJ,Reiken S,Meli AC,Umanskaya A,Xie W,Shiomi T,Zalk R,Lacampagne A,Marks AR||Cell metabolism (14:196)||2011|
iNOS ablation does not improve specific force of the extensor digitorum longus muscle in dystrophin-deficient mdx4cv mice.
MA3-925 was used in western blot to investigate the involvement of inducible nitric oxide synthase in force generation in dystrophin-deficient muscle
|Li D,Shin JH,Duan D||PloS one (6:null)||2011|
Overexpression of transient receptor potential canonical type 1 (TRPC1) alters both store operated calcium entry and depolarization-evoked calcium signals in C2C12 cells.
MA3-925 was used in western blot to study the role of transient receptor potential canonical type 1 (TRPC1) in skeletal muscle
|Oláh T,Fodor J,Ruzsnavszky O,Vincze J,Berbey C,Allard B,Csernoch L||Cell calcium (49:415)||2011|
Identification of novel ryanodine receptor 1 (RyR1) protein interaction with calcium homeostasis endoplasmic reticulum protein (CHERP).
MA3-925 was used in western blot to identify interacting partners of the skeletal muscle receptor, ryanodine receptor 1
|Ryan T,Sharma P,Ignatchenko A,MacLennan DH,Kislinger T,Gramolini AO||The Journal of biological chemistry (286:17060)||2011|
Antioxidant network expression abrogates oxidative posttranslational modifications in mice.
MA3-925 was used in western blot to examine the therapeutic effect of antioxidant enzymes against myocardial ischemia-reperfusion injury
|Mital R,Zhang W,Cai M,Huttinger ZM,Goodman LA,Wheeler DG,Ziolo MT,Dwyer KM,d'Apice AJ,Zweier JL,He G,Cowan PJ,Gumina RJ||American journal of physiology. Heart and circulatory physiology (300:H1960)||2011|
Ageing, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function.
MA3-925 was used in western blot to study the effects of ageing on muscle quality and sarcoplasmic reticulum Ca(2+) release.
|Russ DW,Grandy JS,Toma K,Ward CW||Acta physiologica (Oxford, England) (201:391)||2011|
Extracellular matrix of collagen modulates intracellular calcium handling and electrophysiological characteristics of HL-1 cardiomyocytes with activation of angiotensin II type 1 receptor.
MA3-925 was used in western blot to investigate the involvement of collagen in calcium homeostasis and the electrophysiological features of cardiomyocytes
|Lu YY,Chen YC,Kao YH,Wu TJ,Chen SA,Chen YJ||Journal of cardiac failure (17:82)||2011|
Total beta-adrenoceptor deficiency results in cardiac hypotrophy and negative inotropy.
MA3-925 was used in western blot to investigate the role of beta adrenoceptor in cardiac strutural and functional integrity
|Lee S,Grafweg S,Schneider T,Jimenez M,Giacobino JP,Ghanem A,Tiemann K,Bloch W,Müller-Ehmsen J,Schwinger RH,Brixius K||Physiological research / Academia Scientiarum Bohemoslovaca (59:679)||2010|
Ca2+ overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle.
MA3-925 was used in western blot to investigate the function of trimeric intracellular cation channels in sarcoplasmic reticulum
|Zhao X,Yamazaki D,Park KH,Komazaki S,Tjondrokoesoemo A,Nishi M,Lin P,Hirata Y,Brotto M,Takeshima H,Ma J||The Journal of biological chemistry (285:37370)||2010|
Paclitaxel accelerates spontaneous calcium oscillations in cardiomyocytes by interacting with NCS-1 and the InsP3R.
MA3-925 was used in western blot to investigate the effect of taxol on calcium signaling and calcium oscillation
|Zhang K,Heidrich FM,DeGray B,Boehmerle W,Ehrlich BE||Journal of molecular and cellular cardiology (49:829)||2010|
Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure.
MA3-925 was used in western blot to investigate the effect of aerobic exercise on skeletal muscle function and calcium-related protein expression
|Bueno CR,Ferreira JC,Pereira MG,Bacurau AV,Brum PC||Journal of applied physiology (Bethesda, Md. : 1985) (109:702)||2010|
Role of presenilins in neuronal calcium homeostasis.
MA3-925 was used in western blot to demonstrate that presenilins play an important role in neuronal ER calcium homeostasis
|Zhang H,Sun S,Herreman A,De Strooper B,Bezprozvanny I||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:8566)||2010|
Initiating treadmill training in late middle age offers modest adaptations in Ca2+ handling but enhances oxidative damage in senescent rat skeletal muscle.
MA3-925 was used in western blot to identify the relationship between calcium handling and muscle senescence during late middle-age training
|Thomas MM,Vigna C,Betik AC,Tupling AR,Hepple RT||American journal of physiology. Regulatory, integrative and comparative physiology (298:R1269)||2010|
Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice.
MA3-925 was used in western blot to investigate the effect of aerobic exercise on calcium signal and redox state of skeletal muscle
|Ferreira JC,Bacurau AV,Bueno CR,Cunha TC,Tanaka LY,Jardim MA,Ramires PR,Brum PC||Experimental biology and medicine (Maywood, N.J.) (235:497)||2010|
Abnormalities in intracellular Ca2+ regulation contribute to the pathomechanism of Tako-Tsubo cardiomyopathy.
MA3-925 was used in western blot to investigate the gene expression of calcium-handling proteins in Tako-Tsubo cardiomyopathy
|Nef HM,Möllmann H,Troidl C,Kostin S,Voss S,Hilpert P,Behrens CB,Rolf A,Rixe J,Weber M,Hamm CW,Elsässer A||European heart journal (30:2155)||2009|
Deranged calcium signaling and neurodegeneration in spinocerebellar ataxia type 3.
MA3-925 was used in western blot to investigate the association of mutant ATX3 with the type 1 inositol 1,4,5-trisphosphate receptor and its effect on calcium signalling in spinocerebellar ataxia type 3.
|Chen X,Tang TS,Tu H,Nelson O,Pook M,Hammer R,Nukina N,Bezprozvanny I||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:12713)||2008|
Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle.
MA3-925 was used in western blot to investigate the expression of sarcalumenin and related calcium-regulatory proteins in rodent skeletal muscle during aging
|O'Connell K,Gannon J,Doran P,Ohlendieck K||Experimental gerontology (43:958)||2008|
Disruption of the intracellular Ca2+ homeostasis in the cardiac excitation-contraction coupling is a crucial mechanism of arrhythmic toxicity in aconitine-induced cardiomyocytes.
MA3-925 was used in western blot to investigate the mechanism of arrhythmic cytotoxicity in heart cells treated with aconitine
|Fu M,Wu M,Wang JF,Qiao YJ,Wang Z||Biochemical and biophysical research communications (354:929)||2007|
Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins.
MA3-925 was used in western blot to investigate the important role of junctophilins in neural excitability and plasticity
|Moriguchi S,Nishi M,Komazaki S,Sakagami H,Miyazaki T,Masumiya H,Saito SY,Watanabe M,Kondo H,Yawo H,Fukunaga K,Takeshima H||Proceedings of the National Academy of Sciences of the United States of America (103:10811)||2006|
Increased phospholamban phosphorylation limits the force-frequency response in the MLP-/- mouse with heart failure.
MA3-925 was used in western blot to study the role of increased phospholamban phosphorylation in limiting the force-frequency response in a model of dilated cardiomyopathy
|Antoons G,Vangheluwe P,Volders PG,Bito V,Holemans P,Ceci M,Wuytack F,Caroni P,Mubagwa K,Sipido KR||Journal of molecular and cellular cardiology (40:350)||2006|
Further study on the role of HSP70 on Ca2+ homeostasis in rat ventricular myocytes subjected to simulated ischemia.
MA3-925 was used in western blot to investigate the role of HSP70 on calcium homeostasis in rat ventricular myocytes subjected to simulated ischemia
|Liu J,Kam KW,Borchert GH,Kravtsov GM,Ballard HJ,Wong TM||American journal of physiology. Cell physiology (290:C583)||2006|
Calcium release from ryanodine receptors in the nucleoplasmic reticulum.
MA3-925 was used in western blot to investigate the presence of RyR in the intranuclear extensions of nuclear envelope and its role in calcium signaling
|Marius P,Guerra MT,Nathanson MH,Ehrlich BE,Leite MF||Cell calcium (39:65)||2006|
Expression of the skeletal muscle dystrophin-dystroglycan complex and syntrophin-nitric oxide synthase complex is severely affected in the type 2 diabetic Goto-Kakizaki rat.
MA3-925 was used in western blot to study the reduced expression of the dystrophin-dystroglycan complex and the syntrophin-NOS complex in skeletal muscle of type 2 diabetic rats and the significance for insulin resistance
|Mulvey C,Harno E,Keenan A,Ohlendieck K||European journal of cell biology (84:867)||2005|
Organic cation permeation through the channel formed by polycystin-2.
MA3-925 was used in western blot to characterize the channels formed by a member of the transient receptor potential family of ion channels polycystin-2.
|Anyatonwu GI,Ehrlich BE||The Journal of biological chemistry (280:29488)||2005|
Mechanical unloading improves intracellular Ca2+ regulation in rats with doxorubicin-induced cardiomyopathy.
MA3-925 was used in western blot to investigate the effects of mechanical unloading on cardiomyocytes from doxorubicin-induced cardiomyopathy
|Takaseya T,Ishimatsu M,Tayama E,Nishi A,Akasu T,Aoyagi S||Journal of the American College of Cardiology (44:2239)||2004|
Cardiac adenoviral S100A1 gene delivery rescues failing myocardium.
MA3-925 was used in western blot to investigaet the ability of S100A2 gene transfer to rescue failing myocardium
|Most P,Pleger ST,Völkers M,Heidt B,Boerries M,Weichenhan D,Löffler E,Janssen PM,Eckhart AD,Martini J,Williams ML,Katus HA,Remppis A,Koch WJ||The Journal of clinical investigation (114:1550)||2004|
TRPC3 channels confer cellular memory of recent neuromuscular activity.
MA3-925 was used in western blot to study the regulation of NFAT functions through neuromuscular stimulation in rodent skeletal muscles.
|Rosenberg P,Hawkins A,Stiber J,Shelton JM,Hutcheson K,Bassel-Duby R,Shin DM,Yan Z,Williams RS||Proceedings of the National Academy of Sciences of the United States of America (101:9387)||2004|
Location of divergent region 2 on the three-dimensional structure of cardiac muscle ryanodine receptor/calcium release channel.
MA3-925 was used in western blot to study the 3-dimensional localisation of divergent region 2 of the cardiac muscle ryanodine receptor/calcium release channel
|Liu Z,Zhang J,Wang R,Wayne Chen SR,Wagenknecht T||Journal of molecular biology (338:533)||2004|
The 12 kDa FK506-binding protein, FKBP12, modulates the Ca(2+)-flux properties of the type-3 ryanodine receptor.
MA3-925 was used in western blot to study the effect of FKBP12 on the activity of the type-3 ryanodine receptor.
|Van Acker K,Bultynck G,Rossi D,Sorrentino V,Boens N,Missiaen L,De Smedt H,Parys JB,Callewaert G||Journal of cell science (117:1129)||2004|
S-glutathionylation decreases Mg2+ inhibition and S-nitrosylation enhances Ca2+ activation of RyR1 channels.
MA3-925 was used in western blot to investigate the effect of S-glutathionylation and S-nitrosylation on the modulation of RyR1 channels by Mg2+ and calcium.
|Aracena P,Sánchez G,Donoso P,Hamilton SL,Hidalgo C||The Journal of biological chemistry (278:42927)||2003|
Transgenic overexpression of the Ca2+-binding protein S100A1 in the heart leads to increased in vivo myocardial contractile performance.
MA3-925 was used in western blot to investigate the role of S100A1 in the regulation of cardiac function in vivo
|Most P,Remppis A,Pleger ST,Löffler E,Ehlermann P,Bernotat J,Kleuss C,Heierhorst J,Ruiz P,Witt H,Karczewski P,Mao L,Rockman HA,Duncan SJ,Katus HA,Koch WJ||The Journal of biological chemistry (278:33809)||2003|
Defects in ryanodine receptor calcium release in skeletal muscle from post-myocardial infarct rats.
MA3-925 was used in western blot to investigate the relationship between ryanodine receptor calcium release and impaired myocardial function
|Ward CW,Reiken S,Marks AR,Marty I,Vassort G,Lacampagne A||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (17:1517)||2003|
Isoform-dependent formation of heteromeric Ca2+ release channels (ryanodine receptors).
MA3-925 was used in western blot to investigate the function of three ryanodine receptor isoforms during the formation of the functional heteromeric channels.
|Xiao B,Masumiya H,Jiang D,Wang R,Sei Y,Zhang L,Murayama T,Ogawa Y,Lai FA,Wagenknecht T,Chen SR||The Journal of biological chemistry (277:41778)||2002|
Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats.
MA3-925 was used in western blot to study the possible contribution of defects in intracellular calcium signaling cardiomyopathy in streptozotocin-induced diabetic rats
|Choi KM,Zhong Y,Hoit BD,Grupp IL,Hahn H,Dilly KW,Guatimosim S,Lederer WJ,Matlib MA||American journal of physiology. Heart and circulatory physiology (283:H1398)||2002|
Cardiac remodeling and atrial fibrillation in transgenic mice overexpressing junctin.
MA3-925 was used in western blot to study junctin's functions in heart
|Hong CS,Cho MC,Kwak YG,Song CH,Lee YH,Lim JS,Kwon YK,Chae SW,Kim DH||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (16:1310)||2002|
Molecular basis of Ca(2)+ activation of the mouse cardiac Ca(2)+ release channel (ryanodine receptor).
MA3-925 was used in western blot to study the mechanism for calcium activation of the mouse cardiac ryanodine receptor.
|Li P,Chen SR||The Journal of general physiology (118:33)||2001|
Contraction and intracellular Ca(2+) handling in isolated skeletal muscle of rats with congestive heart failure.
MA3-925 was used in western blot to study the contraction and intracellular calcium handling in rat skeletal muscle
|Lunde PK,Dahlstedt AJ,Bruton JD,Lännergren J,Thorén P,Sejersted OM,Westerblad H||Circulation research (88:1299)||2001|
Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus.
MA3-925 was used in western blot to investigate the Three-dimensional structure of the recombinant type 3 ryanodine receptor
|Liu Z,Zhang J,Sharma MR,Li P,Chen SR,Wagenknecht T||Proceedings of the National Academy of Sciences of the United States of America (98:6104)||2001|
Enhanced sarcoplasmic reticulum Ca(2+) release following intermittent sprint training.
MA3-925 was used in western blot to study how intermittent sprint training affects sarcoplasmic reticulum functions
|Ortenblad N,Lunde PK,Levin K,Andersen JL,Pedersen PK||American journal of physiology. Regulatory, integrative and comparative physiology (279:R152)||2000|
Ca2+-induced Ca2+ release supports the relay mode of activity in thalamocortical cells.
MA3-925 was used in western blot to investigate the mechanism for the calcium-induced calcium release in thalamocortical cells
|Budde T,Sieg F,Braunewell KH,Gundelfinger ED,Pape HC||Neuron (26:483)||2000|
Isometric force and endurance in soleus muscle of thyroid hormone receptor-alpha(1)- or -beta-deficient mice.
MA3-925 was used in western blot to investigate the role of thyroid hormone receptor (TR)-alpha1 and -beta on skeletal muscle function
|Johansson C,Lännergren J,Lunde PK,Vennström B,Thorén P,Westerblad H||American journal of physiology. Regulatory, integrative and comparative physiology (278:R598)||2000|
Restoration of diastolic function in senescent rat hearts through adenoviral gene transfer of sarcoplasmic reticulum Ca(2+)-ATPase.
MA3-925 was used in western blot to investigate the possible relationship between the restoration of the SR calcium-ATPase through adenoviral gene transfer in the aging heart and the normalization of diastolic function in vivo
|Schmidt U,del Monte F,Miyamoto MI,Matsui T,Gwathmey JK,Rosenzweig A,Hajjar RJ||Circulation (101:790)||2000|
Further characterization of the type 3 ryanodine receptor (RyR3) purified from rabbit diaphragm.
MA3-925 was used in western blot to study the structure and function of type 3 ryanodine receptor (RyR3) in rabbit diaphragm
|Murayama T,Oba T,Katayama E,Oyamada H,Oguchi K,Kobayashi M,Otsuka K,Ogawa Y||The Journal of biological chemistry (274:17297)||1999|
Skeletal muscle type ryanodine receptor is involved in calcium signaling in human B lymphocytes.
MA3-925 was used in western blot to study the role of skeletal muscle type RYR in calcium signaling in human B lymphocytes
|Sei Y,Gallagher KL,Basile AS||The Journal of biological chemistry (274:5995)||1999|
Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum.
MA3-925 was used in western blot to investigate the major tryptic fragments of RyR and their relationships with the insoluble membrane fraction and sedimentation behavior
|Chen SR,Airey JA,MacLennan DH||The Journal of biological chemistry (268:22642)||1993|
A unique ion channel clustering domain on the axon initial segment of mammalian neurons.
MA3-925 was used in immunohistochemistry - free floating to study Kv2.1 function and membrane excitability in the axon initial segment
|King AN,Manning CF,Trimmer JS||The Journal of comparative neurology (522:2594)||2014|
Bcl-2 binds to and inhibits ryanodine receptors.
MA3-925 was used in immunoprecipitation and western blot to study the inhibition of ryanodine recptors by Bcl-2
|Vervliet T,Decrock E,Molgó J,Sorrentino V,Missiaen L,Leybaert L,De Smedt H,Kasri NN,Parys JB,Bultynck G||Journal of cell science (127:2782)||2014|
Modulation of Ca(2+) release through ryanodine receptors in vascular smooth muscle by protein kinase Calpha.
MA3-925 was used in immunoprecipitation to study the role of PKC alpha in ryanodine receptor-mediated calcium release in vascular smooth muscle
|Peng H,Yaney GC,Kirber MT||Pflu¿gers Archiv : European journal of physiology (460:791)||2010|
Increased sarcoplasmic reticulum calcium leak but unaltered contractility by acute CaMKII overexpression in isolated rabbit cardiac myocytes.
MA3-925 was used in immunoprecipitation to study the role of acute CaMKII overexpression in calcium leakage and contractility in rabbit cardiomyocytes
|Kohlhaas M,Zhang T,Seidler T,Zibrova D,Dybkova N,Steen A,Wagner S,Chen L,Brown JH,Bers DM,Maier LS||Circulation research (98:235)||2006|
S100A1 increases the gain of excitation-contraction coupling in isolated rabbit ventricular cardiomyocytes.
MA3-925 was used in immunoprecipitation to investigate the effect of s100A1 on the excitation-contraction coupling in isolated rabbit ventricular cardiomyocytes
|Kettlewell S,Most P,Currie S,Koch WJ,Smith GL||Journal of molecular and cellular cardiology (39:900)||2005|
Protein kinase A phosphorylation of the ryanodine receptor does not affect calcium sparks in mouse ventricular myocytes.
MA3-925 was used in immunoprecipitation to study the RyR phosphorylation in PLB-KO mouse ventricular myocytes
|Li Y,Kranias EG,Mignery GA,Bers DM||Circulation research (90:309)||2002|
Identification of a 97-kDa mastoparan-binding protein involving in Ca(2+) release from skeletal muscle sarcoplasmic reticulum.
MA3-925 was used in immunoprecipitation to investigate the function of mastoparan in heavy fraction of SR of rabbit skeletal muscles
|Hirata Y,Nakahata N,Ohizumi Y||Molecular pharmacology (57:1235)||2000|
Distinct regions of triadin are required for targeting and retention at the junctional domain of the sarcoplasmic reticulum.
MA3-925 was used in immunocytochemistry to study three regions of triadin that are involved in its targeting to the sarcoplasmic reticulum junctional domain
|Rossi D,Bencini C,Maritati M,Benini F,Lorenzini S,Pierantozzi E,Scarcella AM,Paolini C,Protasi F,Sorrentino V||The Biochemical journal (458:407)||2014|
Sorcin links calcium signaling to vesicle trafficking, regulates Polo-like kinase 1 and is necessary for mitosis.
MA3-925 was used in immunocytochemistry to study the intracellular role of sorcin.
|Lalioti VS,Ilari A,O'Connell DJ,Poser E,Sandoval IV,Colotti G||PloS one (9:null)||2014|
Evoked centripetal Ca(2+) mobilization in cardiac Purkinje cells: insight from a model of three Ca(2+) release regions.
MA3-925 was used in immunocytochemistry to study Ca(2+) handling in Purkinje cells
|Haq KT,Daniels RE,Miller LS,Miura M,ter Keurs HE,Bungay SD,Stuyvers BD||The Journal of physiology (591:4301)||2013|
PI3Ks maintain the structural integrity of T-tubules in cardiac myocytes.
MA3-925 was used in immunocytochemistry to investigate the importance of phosphoinositide 3-kinases in the organization and structure of cardiomyocytic T-tubules
|Wu CY,Jia Z,Wang W,Ballou LM,Jiang YP,Chen B,Mathias RT,Cohen IS,Song LS,Entcheva E,Lin RZ||PloS one (6:null)||2011|
|Human||Not Cited||Enhanced excitation-coupled Ca(2+) entry induces nuclear translocation of NFAT and contributes to IL-6 release from myotubes from patients with central core disease.||Treves S,Vukcevic M,Jeannet PY,Levano S,Girard T,Urwyler A,Fischer D,Voit T,Jungbluth H,Lillis S,Muntoni F,Quinlivan R,Sarkozy A,Bushby K,Zorzato F||Human molecular genetics (20:589)||2011|
Distribution of ryanodine receptors in rat ventricular myocytes.
MA3-925 was used in immunocytochemistry to investigate the expression of ryanodine receptors in ventricular myocytes
|Salnikov V,Lukyanenko YO,Lederer WJ,Lukyanenko V||Journal of muscle research and cell motility (30:161)||2009|
Mobilization of calcium from intracellular stores facilitates somatodendritic dopamine release.
MA3-925 was used in immunocytochemistry to investigate the effects of calcium release from intracellular stores on somatodendritic DA release in SNc
|Patel JC,Witkovsky P,Avshalumov MV,Rice ME||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:6568)||2009|
Anatomical and neurochemical characterization of dopaminergic interplexiform processes in mouse and rat retinas.
MA3-925 was used in immunocytochemistry to study the organization of dopaminergic interplexiform processes in the outer layer of rodent retinas and possible corresponding functions
|Witkovsky P,Gábriel R,Krizaj D||The Journal of comparative neurology (510:158)||2008|
Functional sarcoplasmic reticulum for calcium handling of human embryonic stem cell-derived cardiomyocytes: insights for driven maturation.
MA3-925 was used in immunocytochemistry to investigate the role of functional sarcoplasmic reticulum in maturation of cardiomyocytes
|Liu J,Fu JD,Siu CW,Li RA||Stem cells (Dayton, Ohio) (25:3038)||2007|
Nitric oxide regulates growth cone filopodial dynamics via ryanodine receptor-mediated calcium release.
MA3-925 was used in immunocytochemistry to study the role of the ryanodine receptor in the regulation of growth cone filopodial dynamics by nitric oxide
|Welshhans K,Rehder V||The European journal of neuroscience (26:1537)||2007|
Ca2+ sparks and waves in canine purkinje cells: a triple layered system of Ca2+ activation.
MA3-925 was used in immunocytochemistry to study the role of Ca2+ sparks and waves in canine Purkinje cells
|Stuyvers BD,Dun W,Matkovich S,Sorrentino V,Boyden PA,ter Keurs HE||Circulation research (97:35)||2005|
Inhibition of ryanodine receptor 1 in fast skeletal muscle fibers induces a fast-to-slow muscle fiber type transition.
MA3-925 was used in immunocytochemistry to demonstrate the effect of ryanodine receptor 1 on muscle fiber type specification.
|Jordan T,Jiang H,Li H,DiMario JX||Journal of cell science (117:6175)||2004|
RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets.
MA3-925 was used in immunocytochemistry to investigate the role of RyR2 in beta-cell survival in vitro and its mechanism .
|Johnson JD,Han Z,Otani K,Ye H,Zhang Y,Wu H,Horikawa Y,Misler S,Bell GI,Polonsky KS||The Journal of biological chemistry (279:24794)||2004|
Activation of metabotropic glutamate receptor mGlu5 on nuclear membranes mediates intranuclear Ca2+ changes in heterologous cell types and neurons.
MA3-925 was used in immunocytochemistry to suggest a role for metabotropic glutamate receptor mGlu5 in intranuclear calcium signaling pathways.
|O'Malley KL,Jong YJ,Gonchar Y,Burkhalter A,Romano C||The Journal of biological chemistry (278:28210)||2003|
Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons.
MA3-925 was used in immunocytochemistry to study the distribution of Kv2.1 channels in developing rat hippocampal neurons
|Antonucci DE,Lim ST,Vassanelli S,Trimmer JS||Neuroscience (108:69)||2001|
Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells.
MA3-925 was used in immunocytochemistry to investigate the translational diffusion of globular proteins in the cytoplasm of cultured muscle cells
|Arrio-Dupont M,Foucault G,Vacher M,Devaux PF,Cribier S||Biophysical journal (78:901)||2000|
Overexpression of histidine-rich calcium binding protein in equine ventricular myocardium.
MA3-925 was used in immunohistochemistry to investigate myocardic histidine-rich calcium binding protein in horses
|Sacchetto R,Sharova E,Patruno M,Maccatrozzo L,Damiani E,Mascarello F||Veterinary journal (London, England : 1997) (193:157)||2012|
Unmasking potential intracellular roles for dysferlin through improved immunolabeling methods.
MA3-925 was used in immunohistochemistry to investigate the localization of dysferlin in skeletal muscle through optimized immunolabeling methods
|Roche JA,Ru LW,O'Neill AM,Resneck WG,Lovering RM,Bloch RJ||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (59:964)||2011|
Calsequestrin and junctin immunoreactivity in hexagonally cross-linked tubular arrays myopathy.
MA3-925 was used in immunohistochemistry to investigate the immunohistochemical features of hexagonally cross-linked tubular arrays myopathy
|Di Blasi C,Blasevich F,Bellafiore E,Mottarelli E,Gibertini S,Zanotti S,Saredi S,Mantegazza R,Morandi L,Mora M||Neuromuscular disorders : NMD (20:326)||2010|
Tumor necrosis factor potentiates central vagal afferent signaling by modulating ryanodine channels.
MA3-925 was used in immunohistochemistry to investigate the role of the tumor necrosis factor during the central vagal afferent signaling process.
|Rogers RC,Van Meter MJ,Hermann GE||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:12642)||2006|
The origin of tubular aggregates in human myopathies.
MA3-925 was used in immunohistochemistry to study the origin and significance of tubular aggregates in human myopathies
|Chevessier F,Bauché-Godard S,Leroy JP,Koenig J,Paturneau-Jouas M,Eymard B,Hantaï D,Verdière-Sahuqué M||The Journal of pathology (207:313)||2005|
Effects of ryanodine receptor activation on neurotransmitter release and neuronal cell death following kainic acid-induced status epilepticus.
MA3-925 was used in immunohistochemistry to investigate the molecular mechanism responsible for kainic acid-induced neuronal cell death
|Mori F,Okada M,Tomiyama M,Kaneko S,Wakabayashi K||Epilepsy research (65:59)||2005|
Neuregulin receptor ErbB2 localization at T-tubule in cardiac and skeletal muscle.
MA3-925 was used in immunohistochemistry to locate neuregulin receptor ErbB2 at T-tubule in cardiac and skeletal muscle
|Ueda H,Oikawa A,Nakamura A,Terasawa F,Kawagishi K,Moriizumi T||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (53:87)||2005|
Nitric oxide synthase in cardiac sarcoplasmic reticulum.
MA3-925 was used in immunohistochemistry to study the localization and function of the nitric oxide synthase in heart.
|Xu KY,Huso DL,Dawson TM,Bredt DS,Becker LC||Proceedings of the National Academy of Sciences of the United States of America (96:657)||1999|
Cellular distribution of Ca2+ pumps and Ca2+ release channels in rat cardiac hypertrophy induced by aortic stenosis.
MA3-925 was used in immunohistochemistry to study the cellular distribution of RyR in rat cardiac hypertrophy
|Anger M,Lompré AM,Vallot O,Marotte F,Rappaport L,Samuel JL||Circulation (98:2477)||1998|
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle.
MA3-925 was used in immunohistochemistry to investigate the calcium release apparatus in skeletal muscle
|Protasi F,Franzini-Armstrong C,Allen PD||The Journal of cell biology (140:831)||1998|
calcium release channel; central core disease of muscle; protein phosphatase 1, regulatory subunit 137; ryanodine receptor 1; ryanodine receptor 1 (skeletal); ryanodine receptor 1, skeletal muscle; ryanodine receptor 1-like, skeletal muscle; RyR; sarcoplasmic reticulum calcium release channel; skeletal muscle calcium release channel; skeletal muscle ryanodine receptor; skeletal muscle-type ryanodine receptor; type 1 ryanodine receptor; type 1-like ryanodine receptor
AI528790; BOS_17377; CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; RYR1; Ryr1l; SKRR