|Tested species reactivity||Bovine, Dog, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodine receptor.|
|Contains||0.01% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/500|
|Immunohistochemistry (Frozen) (IHC (F))||1/100|
|Western Blot (WB)||1/100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For immunofluorescent applications, visualization by confocal microscopy is required. Detection by standard fluorescent microscopy will not be adequate to detect the RyR. Additionally, fluorescent, and not enzymatic, detection is required. Due to the intensity of confocal lasers, the use of an anti-fading agent such as DABCO is strongly recommended.
Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Variable t-tubule organization and Ca2+ homeostasis across the atria.
MA1-83782 was used in immunocytochemistry to study the effect of regional t-tubule density during cardiomyocyte Ca(2+) homeostasis
|Frisk M,Koivumäki JT,Norseng PA,Maleckar MM,Sejersted OM,Louch WE||American journal of physiology. Heart and circulatory physiology (307:H609)||2014|
Gamma-enolase and glial fibrillary acidic protein in nervous system tumors. An immunohistochemical study using specific monoclonal antibodies.
MA1-83782 was used in immunohistochemistry to investigate the expression of GFAP and gamma-enolase in nervous system tumors
|Cras P,Martin JJ,Gheuens J||Acta neuropathologica (75:377)||1988|