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|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic Peptide: K(1) E T A A A K F E R Q H M D S(15) C|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 µg/ml|
|Immunohistochemistry (IHC)||10 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody detects the S-peptide in human samples.
MA1-981 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot this antibody detects the S-peptide in K562 cells. In immunohistochemistry, and immunofluorescence procedures MA1-981 recognizes the S-peptide in transfected K562 cells.
The MA1-981 immunogen corresponds to the S-peptide conjugated through a C-terminal cysteine to keyhole limpet hemocyanin.
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene.
Several advantages exist with using the anti S-Peptide epitope tag (S-Tag (TM)). It can be used for numerous applications which include the analysis of localization, expression detection, and purification of polypeptides from mammalian cells. The small size of the tag (15 aa), the ability to purify tagged polypeptides without antibodies, and the very low expression of endogenous tissues in mammalian cells that react with S-Peptide make the S-Peptide antibody an attractive alternative to many existing epitope tags.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Coordination of DNA replication and histone modification by the Rik1-Dos2 complex.
MA1-981 was used in western blot to investigate the important role of the Rik1-Dos2 complex in DNA replication and histone modification
|Li F,Martienssen R,Cande WZ||Nature (475:244)||2011|