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Immunofluorescent analysis of S100 Protein (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a S100 Protein monoclonal antibody (Product # MA5-12969) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Rat, Non-human primate, Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified bovine brain S100 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12969 targets S100 Protein in IHC (P), ICC/IF and WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.
The MA5-12969 immunogen is purified bovine brain S100 protein.
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neurogenic contraction induced by the antiarrhythmic compound, AVE 0118, in rat small mesenteric arteries.
MA5-12969 was used in immunohistochemistry to study the effects of an arrhythmic inhibitor of K(+) channels on small mesenteric artery neurogenic contraction
|Kun A,Seprényi G,Varró A,Papp JG,Pataricza J||Basic & clinical pharmacology & toxicology (115:315)||2014|
Perineural invasion and expression of nerve growth factor can predict the progression and prognosis of oral tongue squamous cell carcinoma.
MA5-12969 was used in immunohistochemistry to study the prognostic and predictive value of perineural invasion and NGF expression in patients with oral tongue squamous cell carcinoma
|Shen WR,Wang YP,Chang JY,Yu SY,Chen HM,Chiang CP||Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (43:258)||2014|
Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.
MA5-12969 was used in immunohistochemistry to study the cellular composition of two different biomaterials seeded with chondrocytes and the quality of the cartilage generated following human transplantation
|Horák M,Handl M,Podškubka A,Kaňa R,Adler J,Povýšil C||Folia biologica (60:1)||2014|
A rare malignancy of the parotid gland in a 13-year-old Taiwanese boy: case report of a mammary analogue secretory carcinoma of the salivary gland with molecular study.
MA5-12969 was used in immunohistochemistry to report on a case of mammary analogue secretory carcinoma of the salivary gland
|Hwang MJ,Wu PR,Chen CM,Chen CY,Chen CJ||Medical molecular morphology (47:57)||2014|
Neurophysiological and immunohistochemical studies of IgG anti-GM1 monoclonal antibody on neuromuscular transmission: effects in rat neuromuscular junctions.
MA5-12969 was used in immunohistochemistry to study the effects of an anti-GM1 monoclonal antibody on the rat neuromuscular junction and neuromuscular transmission
|Hotta S,Nagaoka T,Taguchi K,Nakatani Y,Utsnomiya I,Masuda Y,Abe K,Yuki N||Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology (35:205)||2014|
Differential AKT dependency displayed by mouse models of BRAFV600E-initiated melanoma.
MA5-12969 was used in immunohistochemistry to study the role of PI3 kinase signaling in murine models of BRAFV600E-driven melanoma
|Marsh Durban V,Deuker MM,Bosenberg MW,Phillips W,McMahon M||The Journal of clinical investigation (123:5104)||2013|
The sensory innervation of the human pharynx: searching for mechanoreceptors.
MA5-12969 was used in immunohistochemistry to investigate the presence of mechanoreceptors in the human pharynx
|de Carlos F,Cobo J,Macías E,Feito J,Cobo T,Calavia MG,García-Suárez O,Vega JA||Anatomical record (Hoboken, N.J. : 2007) (296:1735)||2013|
Contribution of DOG1 expression to the diagnosis of gastrointestinal stromal tumors.
MA5-12969 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of DOG1 in gastrointestinal stromal tumors
|Kara T,Serinsoz E,Arpaci RB,Gubur O,Orekici G,Ata A,Colak T,Arican A||Pathology, research and practice (209:413)||2013|
Dural angioleiomyoma of the middle cranial fossa: a case report and review of the literature.
MA5-12969 was used in immunohistochemistry to report on a case of dural angioleiomyoma of the middle cranial fossa
|Zhou Z,Yu M,Yang S,Zhou J,Sun R,Yang G||Brain tumor pathology (30:117)||2013|
Concha bullosa surgery and the distribution of human olfactory neuroepithelium.
MA5-12969 was used in immunohistochemistry to study the distribution of olfactory neuroepithelium in patients undergoing concha bullosa surgery
|Apuhan T,Yildirim YS,Simşek T,Yilmaz F,Yilmaz F||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (270:953)||2013|
Evidence of nestin-positive cells in the human cutaneus Meissner and Pacinian corpuscles.
MA5-12969 was used in immunohistochemistry to examine human cutaneus Meissner and Pacinian corpuscles for the presence of nestin-positive cells with the potential to be neural stem cells
|Calavia M,Viña E,Menéndez-González M,López-Muñiz A,Alonso-Guervós M,Cobo J,Otero J,Arias-Carrión O,Vega JA,García-Suárez O||CNS & neurological disorders drug targets (11:869)||2012|
Pigmented paraganglioma of the kidney: a case report.
MA5-12969 was used in immunohistochemistry to report on a case of pigmented paraganglioma of the kidney
|Zhao L,Luo J,Zhang H,Da J||Diagnostic pathology (7:null)||2012|
Histological assessment of mechanoreceptors in Achilles allografts after anterior cruciate ligament reconstruction.
MA5-12969 was used in immunohistochemistry to perform a histological study of mechanoreceptors in reconstructive anterior cruciate ligament allografts
|Kim SH,Chun CH,Chun KC,Jo HJ,Kim KM||The American journal of sports medicine (40:2061)||2012|
|Non-human primate||Not Cited||
Immunohistochemical localization of acid-sensing ion channel 2 (ASIC2) in cutaneous Meissner and Pacinian corpuscles of Macaca fascicularis.
MA5-12969 was used in immunohistochemistry to study the localization of acid-sensing ion channel 2 in cutaneous Meissner and Pacinian corpuscles
|Cabo R,Gálvez MA,San José I,Laurà R,López-Muñiz A,García-Suárez O,Cobo T,Insausti R,Vega JA||Neuroscience letters (516:197)||2012|
Intraglomerular crescentic metastases of malignant melanoma.
MA5-12969 was used in immunohistochemistry to report on a case of intraglomerular crescentic metastases of malignant melanoma
|Ozluk Y,Kilicaslan I,Kazancioglu R,Demirkesen C,Derin D,Mandel NM||Human pathology (42:2025)||2011|
Cotyledonoid leiomyoma of uterus: a case report.
MA5-12969 was used in immunohistochemistry to report on a case of cotyledonoid leiomyoma of uterus
|Ersöz S,Turgutalp H,Mungan S,Güvendı G,Güven S||Tu¿rk patoloji dergisi (27:257)||2011|
Papillary urothelial carcinoma of the bladder with exuberant pseudosarcomatous stromal reaction following radiation therapy.
MA5-12969 was used in immunohistochemistry to report on a case of papillary urothelial carcinoma of the bladder with exuberant pseudosarcomatous stromal reaction
|Ambrosini-Spaltro A,Melissari M||International journal of surgical pathology (19:263)||2011|
Copy number alterations and expression profiles of candidate genes in a pulmonary inflammatory myofibroblastic tumor.
MA5-12969 was used in immunohistochemistry to study the pathogenic mechanisms of pulmonary inflammatory myofibroblastic tumor
|Jung SH,Yim SH,Hu HJ,Jung CK,Lee SH,Kim DH,Chung YJ||Lung cancer (Amsterdam, Netherlands) (70:152)||2010|
Differential localization of Acid-sensing ion channels 1 and 2 in human cutaneus pacinian corpuscles.
MA5-12969 was used in immunohistochemistry to study the expression of acid-sensing ion channels 1, 2 and 3 in human cutaneous pacinian corpuscles
|Calavia MG,Montaño JA,García-Suárez O,Feito J,Guervós MA,Germanà A,Del Valle M,Pérez-Piñera P,Cobo J,Vega JA||Cellular and molecular neurobiology (30:841)||2010|
Extracellular matrix degradation and reduced neural density in children with intrinsic ureteropelvic junction obstruction.
MA5-12969 was used in immunohistochemistry to study nerve density and the expression of proteins involved in ECM degradation in children suffering from intrinsic uteropelvic junction obstruction
|Kaya C,Bogaert G,de Ridder D,Schwentner C,Fritsch H,Oswald J,Radmayr C||Urology (76:185)||2010|
Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?
MA5-12969 was used in immunohistochemistry to report on two cases of giant cell tumor-like lesion of the urinary bladder
|Behzatoğlu K,Durak H,Canberk S,Aydin O,Huq GE,Oznur M,Ozyalvaçli G,Yildiz P||Diagnostic pathology (4:null)||2010|
Rhabdomyosarcoma arising in a mature cystic teratoma with contralateral serous carcinoma: case report and review of the literature.
MA5-12969 was used in immunohistochemistry to report on a case of rhabdomyosarcoma arising in a mature cystic teratoma with contralateral serous carcinoma
|Kefeli M,Kandemir B,Akpolat I,Yildirim A,Kokcu A||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (28:372)||2009|
Uterine carcinosarcoma metastatic to the lung as large-cell neuroendocrine carcinoma with synchronous sarcoid granulomatosis.
MA5-12969 was used in immunohistochemistry to report a clinical case of uterine carcinosarcoma with unique features
|Froio E,D'Adda T,Fellegara G,Martella E,Caruana P,Pruneri G,Pesci A,Rindi G||Lung cancer (Amsterdam, Netherlands) (64:371)||2009|
Primary choroid plexus papilloma of the sacral nerve roots.
MA5-12969 was used in immunohistochemistry to report on a case of choroid plexus papilloma of the sacral nerve roots
|Boldorini R,Panzarasa G,Girardi P,Monga G||Journal of neurosurgery. Spine (10:51)||2009|
The coexistence of invasive ductal carcinoma and malignant phyllodes tumor with liposarcomatous and chondrosarcomatous differentiation in the same breast in a post-osteosarcoma case.
MA5-12969 was used in immunohistochemistry to report on a patient with both invasive ductal carcinoma and malignant phyllodes tumor with liposarcomatous and chondrosarcomatous differentiation
|Kefeli M,Yildiz L,Akpolat I,Balci P,Ozen N||Pathology, research and practice (204:919)||2008|
Metastatic malignant melanoma of the uterine cervix: first diagnosed on liquid-based cytology.
MA5-12969 was used in immunohistochemistry to report on a case of metastatic malignant melanoma of the uterine cervix
|Uzüm N,Köse F,Ataoğlu O||Diagnostic cytopathology (36:769)||2008|
Fine needle aspiration cytology of inflammatory myofibroblastic tumour (inflammatory pseudotumour) of the breast: a case report and review of the literature.
MA5-12969 was used in immunohistochemistry to investigate a clinical case of inflammatory myofibroblastic tumour in the breast
|Akbulut M,Gunhan-Bilgen I,Zekioglu O,Duygulu G,Oktay A,Ozdemir N||Cytopathology : official journal of the British Society for Clinical Cytology (18:384)||2007|
Epithelioid leiomyosarcoma with rhabdoid features.
MA5-12969 was used in immunohistochemistry to report on a case of epithelioid leiomyosarcoma with rhabdoid features
|Yorulmaz G,Erdogan G,Pestereli HE,Savas B,Karaveli FS||Wiener klinische Wochenschrift (119:557)||2007|
Myointimoma of the glans penis.
MA5-12969 was used in immunohistochemistry to report on a case of myointimoma of the glans penis
|Vardar E,Gunlusoy B,Arslan M,Kececi S||Pathology international (57:158)||2007|
Low grade myofibroblastic sarcoma of tongue: a case report.
MA5-12969 was used in immunohistochemistry to report on a case of low grade myofibroblastic sarcoma of the tongue
|Laco J,Simáková E,Slezák R,Tucek L,Mottl R,Spacek J,Ryska A||C¿eskoslovenska¿ patologie (42:150)||2006|
Reduction of GFAP induced by long dark rearing is not restricted to visual cortex.
MA5-12969 was used in immunohistochemistry to study the effect of dark rearing on glial fibrilliary acidic protein in different brain regions
|Corvetti L,Aztiria E,Domenici L||Brain research (1067:146)||2006|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA5-12969 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|
Recurrent solitary fibrous tumour in the cerebellopontine angle.
MA5-12969 was used in immunohistochemistry to report a clinical case of solitary fibrous tumour in the cerebellopontine angle
|Bikmaz K,Cosar M,Kurtkaya-Yapicier O,Iplikcioglu AC,Gokduman CA||Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (12:829)||2005|
Pulmonary large cell carcinoma with rhabdoid phenotype.
MA5-12969 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype
|Yilmazbayhan D,Ates LE,Dilege S,Gulluoglu M,Tanju S,Kalayci G||Annals of diagnostic pathology (9:223)||2005|
Analysis of prognostic and immunohistochemical factors in gastrointestinal stromal tumors with malignant potential.
MA5-12969 was used in immunohistochemistry to evaluate the prognostic significance of multiple factors in gastrointestinal stromal tumors with malignant potential
|Ozgüç H,Yilmazlar T,Yerci O,Soylu R,Tümay V,Filiz G,Zorluoglu A||Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (9:418)||2005|
Collagenous fibroma (desmoplastic fibroblastoma).
MA5-12969 was used in immunohistochemistry to report on a patient with collagenous fibroma
|Dagli M,Eryilmaz A,Acar A,Kulacoglu S,Akmansu H||Yonsei medical journal (45:941)||2004|
Malignant gastrointestinal stromal tumor of the gallbladder.
MA5-12969 was used in immunohistochemistry to report on a case of malignant gastrointestinal stromal tumor of the gallbladder
|Park JK,Choi SH,Lee S,Min KO,Yun SS,Jeon HM||Journal of Korean medical science (19:763)||2004|
Synchronous primary adenocarcinoma and gastrointestinal stromal tumor in the stomach: a report of two cases.
MA5-12969 was used in immunohistochemistry to report on two patients with synchronous primary adenocarcinoma and gastrointestinal stromal tumor in the stomach
|Bircan S,Candir O,Aydin S,Başpinar S,Bülbül M,Kapucuoğlu N,Karahan N,Ciriş M||The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology (15:187)||2004|
Mazabraud's syndrome: intramuscular myxoma associated with fibrous dysplasia.
MA5-12969 was used in immunohistochemistry to report on a case of Mazabraud's syndrome
|Kabukcuoglu F,Kabukcuoglu Y,Yilmaz B,Erdem Y,Evren I||Pathology oncology research : POR (10:121)||2004|
Embryonic development of the ganglion plexuses and the concentric layer structure of human gut: a topographical study.
MA5-12969 was used in immunohistochemistry to study the embryonic development of ganglion plexuses and smooth muscle layers of the human gut
|Fu M,Tam PK,Sham MH,Lui VC||Anatomy and embryology (208:33)||2004|
Two intraligamentary lipomatous tumors with immunohistochemical features.
MA5-12969 was used in immunohistochemistry to report on two cases of two intraligamentary lipomatous tumors
|Cinel L,Düşmez D,Nabaei SH,Taner D,Pata O||Acta obstetricia et gynecologica Scandinavica (81:786)||2002|
Concomitant distribution shift of glial GABA transporter and S100 calcium-binding proteins in the rat retina after kainate-induced excitotoxic injury.
MA5-12969 was used in western blot to study the effect of kainate-induced excitotoxic injury on glial GABA transporter and S100 distribution in rat retina
|Kim D,Kim MJ,Lee JH,Im JO,Won YJ,Yoon SY,Hong HN||Neuroscience letters (353:17)||2003|
alpha polypeptide; protein S100-A1; protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein alpha chain; S-100 protein beta chain; S-100 protein subunit alpha; S-100 protein subunit beta; S100 alpha; S100 calcium-binding protein A1; S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein; S100 protein, alpha polypeptide; S100 protein, beta polypeptide; S100 protein, beta polypeptide, neural
AI266795; AI850290; BOS_1447; Bpb; NEF; S100; S100-alpha; S100-B; S100A; S100A1; S100B; S100beta; S100P